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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2011.03.007
|2 doi
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|a pubmed25n0692.xml
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|a (NLM)21511362
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Yamada, Nana
|e verfasserin
|4 aut
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|a Expression and substrate specificity of betaine/proline transporters suggest a novel choline transport mechanism in sugar beet
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.04.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a GENBANK: AB597033, AB597034
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier GmbH. All rights reserved.
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|a Proline transporters (ProTs) originally described as highly selective transporters for proline, have been shown to also transport glycinebetaine (betaine). Here we examined and compared the transport properties of Bet/ProTs from betaine accumulating (sugar beet, Amaranthus, and Atriplex,) and non-accumulating (Arabidopsis) plants. Using a yeast mutant deficient for uptake of proline and betaine, it was shown that all these transporters exhibited higher affinity for betaine than proline. The uptake of betaine and proline was pH-dependent and inhibited by the proton uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP). We also investigated choline transport by using a choline transport-deficient yeast mutant. Results revealed that these transporters exhibited a higher affinity for choline uptake rather than betaine. Uptake of choline by sugar beet BvBet/ProT1 was independent of the proton gradient and the inhibition by CCCP was reduced compared with that for uptake of betaine, suggesting different proton binding properties between the transport of choline and betaine. Additionally, in situ hybridization experiments revealed the localization of sugar beet BvBet/ProT1 in phloem and xylem parenchyma cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amino Acid Transport Systems, Neutral
|2 NLM
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|a Carrier Proteins
|2 NLM
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|a GABA Plasma Membrane Transport Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Proton Ionophores
|2 NLM
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|a betaine plasma membrane transport proteins
|2 NLM
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|a 146313-33-9
|2 NLM
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|a Betaine
|2 NLM
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|a 3SCV180C9W
|2 NLM
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|a Carbonyl Cyanide m-Chlorophenyl Hydrazone
|2 NLM
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|a 555-60-2
|2 NLM
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|a proline transporter
|2 NLM
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|a 9040-98-6
|2 NLM
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|a Proline
|2 NLM
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|a 9DLQ4CIU6V
|2 NLM
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|a Choline
|2 NLM
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|a N91BDP6H0X
|2 NLM
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|a Sakakibara, Shota
|e verfasserin
|4 aut
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|a Tsutsumi, Koichi
|e verfasserin
|4 aut
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|a Waditee, Rungaroon
|e verfasserin
|4 aut
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|a Tanaka, Yoshito
|e verfasserin
|4 aut
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|a Takabe, Teruhiro
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 14 vom: 15. Sept., Seite 1609-16
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:14
|g day:15
|g month:09
|g pages:1609-16
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|u http://dx.doi.org/10.1016/j.jplph.2011.03.007
|3 Volltext
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|d 168
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|h 1609-16
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