Investigation of the potential for triterpene synthesis in rice through genome mining and metabolic engineering

Investigation of the potential for triterpene synthesis in rice through genome mining and metabolic engineering

© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 191(2011), 2 vom: 16. Juli, Seite 432-448
1. Verfasser: Inagaki, Yoshi-Shige (VerfasserIn)
Weitere Verfasser: Etherington, Graham, Geisler, Katrin, Field, Ben, Dokarry, Melissa, Ikeda, Kousuke, Mutsukado, Yukako, Dicks, Jo, Osbourn, Anne
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2,3-oxidosqualene Plant Proteins Triterpenes Oleanolic Acid 6SMK8R7TGJ Squalene 7QWM220FJH Sterol 14-Demethylase mehr... EC 1.14.14.154 Intramolecular Transferases EC 5.4.- 2,3-oxidosqualene-beta-amyrin-cyclase EC 5.4.99.- lanosterol synthase EC 5.4.99.7 beta-amyrin KM8353IPSO
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245 1 0 |a Investigation of the potential for triterpene synthesis in rice through genome mining and metabolic engineering 
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520 |a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust. 
520 |a The first committed step in sterol biosynthesis in plants involves the cyclization of 2,3-oxidosqualene by the oxidosqualene cyclase (OSC) enzyme cycloartenol synthase. 2,3-Oxidosqualene is also a precursor for triterpene synthesis. Antimicrobial triterpenes are common in dicots, but seldom found in monocots, with the notable exception of oat. Here, through genome mining and metabolic engineering, we investigate the potential for triterpene synthesis in rice. The first two steps in the oat triterpene pathway are catalysed by a divergent OSC (AsbAS1) and a cytochrome P450 (CYP51). The genes for these enzymes form part of a metabolic gene cluster. To investigate the origins of triterpene synthesis in monocots, we analysed systematically the OSC and CYP51 gene families in rice. We also engineered rice for elevated triterpene content. We discovered a total of 12 OSC and 12 CYP51 genes in rice and uncovered key events in the evolution of triterpene synthesis. We further showed that the expression of AsbAS1 in rice leads to the accumulation of the simple triterpene, β-amyrin. These findings provide new insights into the evolution of triterpene synthesis in monocots and open up opportunities for metabolic engineering for disease resistance in rice and other cereals 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a 2,3-oxidosqualene  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Triterpenes  |2 NLM 
650 7 |a Oleanolic Acid  |2 NLM 
650 7 |a 6SMK8R7TGJ  |2 NLM 
650 7 |a Squalene  |2 NLM 
650 7 |a 7QWM220FJH  |2 NLM 
650 7 |a Sterol 14-Demethylase  |2 NLM 
650 7 |a EC 1.14.14.154  |2 NLM 
650 7 |a Intramolecular Transferases  |2 NLM 
650 7 |a EC 5.4.-  |2 NLM 
650 7 |a 2,3-oxidosqualene-beta-amyrin-cyclase  |2 NLM 
650 7 |a EC 5.4.99.-  |2 NLM 
650 7 |a lanosterol synthase  |2 NLM 
650 7 |a EC 5.4.99.7  |2 NLM 
650 7 |a beta-amyrin  |2 NLM 
650 7 |a KM8353IPSO  |2 NLM 
700 1 |a Etherington, Graham  |e verfasserin  |4 aut 
700 1 |a Geisler, Katrin  |e verfasserin  |4 aut 
700 1 |a Field, Ben  |e verfasserin  |4 aut 
700 1 |a Dokarry, Melissa  |e verfasserin  |4 aut 
700 1 |a Ikeda, Kousuke  |e verfasserin  |4 aut 
700 1 |a Mutsukado, Yukako  |e verfasserin  |4 aut 
700 1 |a Dicks, Jo  |e verfasserin  |4 aut 
700 1 |a Osbourn, Anne  |e verfasserin  |4 aut 
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773 1 8 |g volume:191  |g year:2011  |g number:2  |g day:16  |g month:07  |g pages:432-448 
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