|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM207462941 |
| 003 |
DE-627 |
| 005 |
20231224002130.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2011 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la200376f
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0692.xml
|
| 035 |
|
|
|a (DE-627)NLM207462941
|
| 035 |
|
|
|a (NLM)21491852
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Kondyurin, Alexey
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Effect of low molecular weight additives on immobilization strength, activity, and conformation of protein immobilized on PVC and UHMWPE
|
| 264 |
|
1 |
|c 2011
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 30.08.2011
|
| 500 |
|
|
|a Date Revised 10.05.2011
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Horseradish peroxidase (HRP) was immobilized onto both plasticized and unplasticized polyvinylchloride (PVC) and ultrahigh molecular weight polyethylene (UHMWPE). Plasma immersion ion implantation (PIII) in a nitrogen plasma with 20 kV bias was used to facilitate covalent immobilization and to improve the wettability of the surfaces. The surfaces and immobilized protein were studied using attenuated total reflection infrared (ATR-IR) spectroscopy and water contact angle measurements. Protein elution on exposure to repeated sodium dodecyl sulfate (SDS) washing was used to assess the strength of HRP immobilization. The presence of low molecular weight components (plasticizer, additives in solvent, unreacted monomers, adsorbed molecules on surface) was found to have a major influence on the strength of immobilization and the conformation of the protein on the samples not exposed to the PIII treatment. A phenomenological model considering interactions between the low molecular weight components, the protein molecule, and the surface is developed to explain these observations
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Enzymes, Immobilized
|2 NLM
|
| 650 |
|
7 |
|a Polyethylenes
|2 NLM
|
| 650 |
|
7 |
|a Polyvinyls
|2 NLM
|
| 650 |
|
7 |
|a ultra-high molecular weight polyethylene
|2 NLM
|
| 650 |
|
7 |
|a polyvinylidene fluoride
|2 NLM
|
| 650 |
|
7 |
|a 24937-79-9
|2 NLM
|
| 650 |
|
7 |
|a Horseradish Peroxidase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.11.1.-
|2 NLM
|
| 700 |
1 |
|
|a Nosworthy, Neil J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bilek, Marcela M M
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 10 vom: 17. Mai, Seite 6138-48
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:27
|g year:2011
|g number:10
|g day:17
|g month:05
|g pages:6138-48
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la200376f
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 27
|j 2011
|e 10
|b 17
|c 05
|h 6138-48
|