Acoustic microstreaming increases the efficiency of reverse transcription reactions comprising single-cell quantities of RNA
Correlating gene expression with behavior at the single-cell level is difficult, largely because the small amount of available mRNA (<1 pg) degrades before it can be reverse transcribed into a more stable cDNA copy. This study tested the capacity for a novel acoustic microstreaming method ("...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 50(2011), 2 vom: 01. Feb., Seite 116-9
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| 1. Verfasser: |
Boon, Wah Chin
(VerfasserIn) |
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| Weitere Verfasser: |
Petkovic-Duran, Karolina,
White, Kylie,
Tucker, Elena,
Albiston, Anthony,
Manasseh, Richard,
Horne, Malcolm Kenneth,
Aumann, Tim Douglas |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2011
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
DNA, Complementary
RNA
63231-63-0 |
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