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231224s2011 xx ||||| 00| ||chi c |
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|a pubmed25n0691.xml
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|a (DE-627)NLM20729187X
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|a (NLM)21473821
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wu, Li-Xiang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Induction of injury to endothelium of pulmonary artery due to entero superantigen by up regulation of lymphocyte chemokine receptor 5
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| 264 |
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1 |
|c 2011
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 26.01.2012
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| 500 |
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|a Date Revised 08.04.2011
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To observe the injurious effect of T cell activated by Staphylococcus enterotoxin B (SEB) on human pulmonary artery endothelial cell (HPAEC) and explore its possible mechanism
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| 520 |
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|a METHODS: HPAEC was cocultured with SEB-activated T cells supernatant, and the secretion of chemotactic factors from HPAEC was examined. The Transwell inserts was used in chemoattraction assays. After HPAECs were cocultured with T cells and 10 ng/ml SEB for 3 days, HPAEC damage was monitored by microscopy and the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay
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| 520 |
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|a RESULTS: Three kinds of tested chemokines showed a time dependent increase in all supernatant of HPAEC incubated with different concentrations of T cells. After 72 hours, the monocyte chemoattractant protein 1 (MCP 1, ng/ml) in 1×10(-2) , 1×10(-1), 1×10(0) T cell supernatant groups was 1.240±0.103, 4.200±0.305, 6.500±0.500, respectively, macrophage inflammatory protein 1α (MIP 1α, ng/ml) was 0.210±0.015, 0.287±0.012, 0.531±0.037, respectively , and Rantes (ng/ml) was 1.420±0.074, 7.634±0.630, 15.700±1.300, respectively. Rantes presented a two phase secretion mode: in early 6 hours it increased swiftly, but relatively slow at 12, 24, 48, 72 hours. T cell adherent to polycarbonate membrane increased after SEB stimulation in superantigen group compared with control group without SEB stimulation (86.38±14.50 vs. 16.50±2.50, P<0.01). When 10 ng/ml SEB-activated T cell was cocultured with HPAEC, more of originally suspended cultured T cells adhered to HPAEC monolayer [(15.50±1.08)% vs. (1.60±0.22)%, PP<0.01], whereas the cell adhesion ratio decreased markedly in 1 μg/ml Met Rantes group [(4.39±0.66)%, PP<0.01). FACs test of HPAEC adherent T cell showed lymphocyte chemokine receptor 5 (CCR5)/CD4 and CCR5/CD8 increased over 2.5 folds and 2.8 folds compared with 100 ng/ml SEB-activated T cell. Cell death rate of HPAEC was increased when cocultured with SEB-activated T cell in superantigen group compared with HPAEC normal incubation group [(32.50±4.50)% vs. (3.50±0.50)%, P<0.01]
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| 520 |
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|a CONCLUSION: Increased chemoattraction and adherence of SEB-activated T cells to HPAEC could damage HPAEC; this effect was possibly due to up regulation of CCR5 on T cell
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| 650 |
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4 |
|a English Abstract
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a CCL2 protein, human
|2 NLM
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| 650 |
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7 |
|a CCL5 protein, human
|2 NLM
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| 650 |
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7 |
|a Chemokine CCL2
|2 NLM
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| 650 |
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7 |
|a Chemokine CCL3
|2 NLM
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| 650 |
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7 |
|a Chemokine CCL5
|2 NLM
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| 650 |
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7 |
|a Enterotoxins
|2 NLM
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| 650 |
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7 |
|a Receptors, CCR5
|2 NLM
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| 650 |
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7 |
|a Superantigens
|2 NLM
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| 650 |
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7 |
|a enterotoxin B, staphylococcal
|2 NLM
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| 650 |
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7 |
|a 39424-53-8
|2 NLM
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| 700 |
1 |
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|a Xiao, Zheng-Lun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kong, Tian-Han
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 23(2011), 4 vom: 08. Apr., Seite 208-12
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:23
|g year:2011
|g number:4
|g day:08
|g month:04
|g pages:208-12
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2011
|e 4
|b 08
|c 04
|h 208-12
|