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231224s2011 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la200535c
|2 doi
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|a pubmed24n0691.xml
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|a (NLM)21466171
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chen, Chih-Hsin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Improving protein transfer efficiency and selectivity in affinity contact printing by using UV-modified surfaces
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|c 2011
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 11.08.2011
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|a Date Revised 26.04.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Affinity contact printing (αCP) is a technique that allows the selective capture of a target protein from solutions to a polymeric stamp decorated with an antibody, and then the target protein is printed onto a solid surface. The success of αCP critically relies on the precise control of protein-surface interactions. Here, we report a study on the effect of UV on the protein-surface interactions between protein and polydimethylsiloxane stamps and between protein and glass slides decorated with N,N-dimethyl-n-octadecyl-3-aminopropyltrimethoxysilyl chloride (DMOAP). Our results show that UV-modified surfaces can be used to improve the transfer efficiency and selectivity of proteins during αCP. For example, the protein transfer efficiency of human IgG onto a DMOAP-coated slide increases from 7.2% to 45.1% after the UV treatment. On the basis of these results, UV-modified surfaces were employed to develop a αCP system for protein detection. The detection limit of anti-IgG in this system is around 10 ng/mL, and the dynamic range is 4 orders of magnitude
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Dimethylpolysiloxanes
|2 NLM
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| 650 |
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7 |
|a Immobilized Proteins
|2 NLM
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| 650 |
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7 |
|a baysilon
|2 NLM
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| 650 |
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7 |
|a 63148-62-9
|2 NLM
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| 700 |
1 |
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|a Yang, Kun-Lin
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 9 vom: 03. Mai, Seite 5427-32
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:27
|g year:2011
|g number:9
|g day:03
|g month:05
|g pages:5427-32
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| 856 |
4 |
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|u http://dx.doi.org/10.1021/la200535c
|3 Volltext
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