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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2011.02.017
|2 doi
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|a pubmed24n0691.xml
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|a (DE-627)NLM207201315
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|a (NLM)21463950
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Padmanabhan, Priya
|e verfasserin
|4 aut
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|a Suppression subtractive hybridization reveals differential gene expression in sunflower grown in high P
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 06.12.2011
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Masson SAS. All rights reserved.
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|a Sunflower (Helianthus annuus L.) is a commercially important oilseed crop. Previous studies proved that this crop is a promising plant species for phytoextraction of excess soil phosphorus (P) because of its superior P accumulating characteristics. Suppression subtractive hybridization (SSH) strategy was employed to isolate and characterize genes that are induced in response to high P in this crop. SSH library was prepared using cDNA generated from plants treated with high P as the 'tester'. Based on the results of dot blot analysis, 360 positive cDNA clones were selected from the SSH library for sequencing. A total of 89 non-redundant expressed sequence tags (ESTs) were identified as high P-responsive genes and they were classified into 6 functional groups. Several genes involved in metabolism showed markedly preferential expression in the library. For further confirmation, thirteen of the representative ESTs were selected from all categories for RT-PCR analysis and the results showed up-regulation of these genes in response to high P-treatment. The gene expression data derived from this study suggested that several of the up-regulated genes identified under high P-treatment might be involved in P-accumulation and tolerance in this plant
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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|a Phosphorus
|2 NLM
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|a 27YLU75U4W
|2 NLM
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|a Sahi, Shivendra V
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 49(2011), 6 vom: 15. Juni, Seite 584-91
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:49
|g year:2011
|g number:6
|g day:15
|g month:06
|g pages:584-91
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|u http://dx.doi.org/10.1016/j.plaphy.2011.02.017
|3 Volltext
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