Influence of thermal extraction of extracellular polymeric substances on cell integrity in activated sludge and membrane bioreactor samples
The influence of the soluble microbial products (SMP) and extracellular polymeric substances (EPS) heating extraction method on cell viability was evaluated for each phase of the protocol using epifluorescence microscopy. In addition, the effect of different centrifugation conditions (2700 g at 24 d...
| Veröffentlicht in: | Water environment research : a research publication of the Water Environment Federation. - 1998. - 83(2011), 2 vom: 07. Feb., Seite 100-6 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2011
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| Zugriff auf das übergeordnete Werk: | Water environment research : a research publication of the Water Environment Federation |
| Schlagworte: | Journal Article Biopolymers Carbohydrates Membranes, Artificial Proteins Sewage Water 059QF0KO0R |
| Zusammenfassung: | The influence of the soluble microbial products (SMP) and extracellular polymeric substances (EPS) heating extraction method on cell viability was evaluated for each phase of the protocol using epifluorescence microscopy. In addition, the effect of different centrifugation conditions (2700 g at 24 degrees C; 12,000 g at 4 degrees C) was also tested. Sludge samples were collected from a conventional wastewater treatment and a membrane bioreactor (MBR) pilot plant fed in parallel. Results show that different centrifugation parameters do not induce cell membrane damaging. Heating significantly influences membrane integrity; for instance, 75 to 90% of initial viable cells are damaged during this phase, possibly leading to the predominance of protein compared to carbohydrate content. The protein content in EPS is 60 to 88 mg bovine serum albumin/ g volatile suspended solids (VSS); higher values observed in MBR sludge samples are probably attributable to the different characteristics of microbial flocs and process operating parameters. Carbohydrate concentrations are not significantly different regardless of applied procedure and sludge type, and are between 10.4 to 11.6 mg glucose/g VSS |
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| Beschreibung: | Date Completed 25.04.2011 Date Revised 23.09.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1554-7531 |