Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH)

Nitrification of mature sanitary landfill leachate with high-strength of N-NH(4) + (1080-2350 mg L(-1)) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibiti...

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Veröffentlicht in:Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA. - 1991. - 29(2011), 6 vom: 15. Juni, Seite 602-11
1. Verfasser: Yusof, Norjan (VerfasserIn)
Weitere Verfasser: Hassan, Mohd Ali, Yee, Phang Lai, Tabatabaei, Meisam, Othman, Mohd Ridzuan, Mori, Masatsugu, Wakisaka, Minato, Sakai, Kenji, Shirai, Yoshihito
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Quaternary Ammonium Compounds Water Pollutants, Chemical
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520 |a Nitrification of mature sanitary landfill leachate with high-strength of N-NH(4) + (1080-2350 mg L(-1)) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibition before being fed with actual mature leachate. Successful nitrification was achieved with an approximately complete ammonium removal (99%) and 96% of N-NH(4) + conversion to N-NO(-) (3) . The maximum volumetric and specific nitrification rates obtained were 2.56 kg N-NH(4) (+) m(-3) day(-1) and 0.23 g N-NH(4) ( +) g(-1) volatile suspended solid (VSS) day(-1), respectively, at hydraulic retention time (HRT) of 12.7 h and solid retention time of 50 days. Incomplete nitrification was encountered when operating at a higher nitrogen loading rate of 3.14 kg N-NH(4) (+) m(-3) day(-1). The substrate overloading and nitrifiers competition with heterotrophs were believed to trigger the incomplete nitrification. Fluorescence in situ hybridization (FISH) results supported the syntrophic association between the ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria. FISH results also revealed the heterotrophs as the dominant and disintegration of some AOB cell aggregates into single cells which further supported the incomplete nitrification phenomenon 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Quaternary Ammonium Compounds  |2 NLM 
650 7 |a Water Pollutants, Chemical  |2 NLM 
700 1 |a Hassan, Mohd Ali  |e verfasserin  |4 aut 
700 1 |a Yee, Phang Lai  |e verfasserin  |4 aut 
700 1 |a Tabatabaei, Meisam  |e verfasserin  |4 aut 
700 1 |a Othman, Mohd Ridzuan  |e verfasserin  |4 aut 
700 1 |a Mori, Masatsugu  |e verfasserin  |4 aut 
700 1 |a Wakisaka, Minato  |e verfasserin  |4 aut 
700 1 |a Sakai, Kenji  |e verfasserin  |4 aut 
700 1 |a Shirai, Yoshihito  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA  |d 1991  |g 29(2011), 6 vom: 15. Juni, Seite 602-11  |w (DE-627)NLM098164791  |x 1096-3669  |7 nnns 
773 1 8 |g volume:29  |g year:2011  |g number:6  |g day:15  |g month:06  |g pages:602-11 
856 4 0 |u http://dx.doi.org/10.1177/0734242X10397581  |3 Volltext 
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