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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-011-0638-9
|2 doi
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|a eng
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|a Whitfield Åslund, Melissa L
|e verfasserin
|4 aut
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|a 1H NMR metabolomics of earthworm responses to polychlorinated biphenyl (PCB) exposure in soil
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|c 2011
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|a Text
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|a ƒaComputermedien
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|a Date Completed 27.07.2011
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|a Date Revised 20.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a (1)H NMR-based metabolomics was used to examine the metabolic profile of D(2)O-buffer extracted tissues of Eisenia fetida earthworms exposed for 2 days to an artificial soil spiked with sub-lethal concentrations of polychlorinated biphenyls (PCBs) (0, 0.5, 1, 5, 10, or 25 mg/kg Aroclor 1254). Univariate statistical analysis of the identified metabolites revealed a significant increase in ATP concentration in earthworms exposed to the highest soil PCB concentration, but detected no significant changes in other metabolites. However, a multivariate approach which considers alterations in multiple metabolites simultaneously, identified a significant linear relationship between earthworm metabolic profiles and PCB concentration (cross-validated PLS-regression with 7 components, R(2)X = 0.99, R(2)Y = 0.77, Q(2)Y = 0.45, P < 0.001). Significant changes in pair-wise metabolic correlations were also detected as PCB concentration increased. For example, lysine and ATP concentrations showed no apparent correlation in control earthworms (r = 0.22, P = 0.54), but were positively correlated in earthworms from the 25 mg/kg treatment (r = 0.87, P = 0.001). Overall, the observed metabolic responses suggest that PCBs disrupted both carbohydrate (energy) metabolism and membrane (osmolytic) function in E. fetida. The ability of (1)H NMR-based metabolomics to detect these responses suggests that this method offers significant potential for direct assessment of sub-lethal PCB toxicity in soil
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Soil Pollutants
|2 NLM
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|a Polychlorinated Biphenyls
|2 NLM
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|a Simpson, André J
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|a Simpson, Myrna J
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|4 aut
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|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 20(2011), 4 vom: 28. Juni, Seite 836-46
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|x 1573-3017
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|g volume:20
|g year:2011
|g number:4
|g day:28
|g month:06
|g pages:836-46
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