Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 49(2011), 6 vom: 01. Juni, Seite 617-22 |
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| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2011
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Minerals Plant Proteins Uronic Acids Sodium Chloride 451W47IQ8X Sodium Acetate 4550K0SC9B Pectins plus... |
| Résumé: | Copyright © 2011 Elsevier Masson SAS. All rights reserved. Autolysis of cell walls from polygalacturonase (PG)-antisense tomato fruit was studied in a conventional buffer designed to maximize the catalytic activity of PG (30 mM sodium acetate, 150 mM NaCl, pH 4.5), and in solutions mimicking the pH and mineral composition of the fruit apoplast at the mature-green and ripe stages. Autolytic release of uronic acids was very limited under simulated apoplastic conditions compared with the conventional buffer, but minimal differences in the release of reducing groups were observed among the incubation conditions. Autolytic release of uronic acids from active walls was lower than solubilization from enzymically inactive walls. Uronic acids that remained ionically bound to the cell walls during autolysis were subsequently extracted and analyzed by size exclusion chromatography. The elution profiles of ionically bound uronic acids from cell walls incubated under optimal conditions were similar for all ripening stages. In solutions mimicking the pH and mineral composition of the apoplast of mature-green and ripe fruit, uronic acids extracted from pink and ripe fruit cell walls showed a decrease in average molecular mass compared with polymers from mature-green cell walls. The results suggest that the composition of the incubation solution exert strong influence on PG-independent cell wall autolysis and that enzymically active walls restrain PG-independent pectin solubilization |
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| Description: | Date Completed 06.12.2011 Date Revised 07.12.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2011.02.021 |