Non-invasive vibrational SFG spectroscopy reveals that bacterial adhesion can alter the conformation of grafted "brush" chains on SAM

Non-invasive vibrational SFG spectroscopy reveals that bacterial adhesion can alter the conformation of grafted "brush" chains on SAM

© 2011 American Chemical Society

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 27(2011), 8 vom: 19. Apr., Seite 4928-35
1. Verfasser: Bulard, Emilie (VerfasserIn)
Weitere Verfasser: Guo, Ziang, Zheng, Wanquan, Dubost, Henri, Fontaine-Aupart, Marie-Pierre, Bellon-Fontaine, Marie-Noëlle, Herry, Jean-Marie, Briandet, Romain, Bourguignon, Bernard
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Alkanes Sulfhydryl Compounds octadecane N102P6HAIU
Beschreibung
Zusammenfassung:© 2011 American Chemical Society
Understanding bacterial adhesion on a surface is a crucial step to design new materials with improved properties or to control biofilm formation and eradication. Sum Frequency Generation (SFG) vibrational spectroscopy has been employed to study in situ the conformational response of a self-assembled monolayer (SAM) of octadecanethiol (ODT) on a gold film to the adhesion of hydrophilic and hydrophobic ovococcoid model bacteria. The present work highlights vibrational SFG spectroscopy as a powerful and unique non-invasive biophysical technique to probe and control bacteria interaction with ordered surfaces. Indeed, the SFG vibrational spectral changes reveal different ODT SAM conformations in air and upon exposure to aqueous solution or bacterial adhesion. Furthermore, this effect depends on the bacterial cell surface properties. The SFG spectral modeling demonstrates that hydrophobic bacteria flatten the ODT SAM alkyl chain terminal part, whereas the hydrophilic ones raise this ODT SAM terminal part. Microorganism-induced alteration of grafted chains can thus affect the desired interfacial functionality, a result that should be considered for the design of new reactive materials
Beschreibung:Date Completed 26.08.2011
Date Revised 15.11.2012
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la200205e