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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la104964z
|2 doi
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|a pubmed24n0689.xml
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|a (DE-627)NLM20660498X
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|a (NLM)21401056
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Wegerich, Franziska
|e verfasserin
|4 aut
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1 |
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|a Electroactive multilayer assemblies of bilirubin oxidase and human cytochrome C mutants
|b insight in formation and kinetic behavior
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.07.2011
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|a Date Revised 30.03.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Here, we report on cytochrome c/bilirubin oxidase multilayer electrodes with different cytochrome c (cyt c) forms including mutant forms of human cyt c, which exhibit different reaction rates with bilirubin oxidase (BOD) in solution. The multilayer formation via the layer-by-layer technique and the kinetic behavior of the mono (only cyt c) and biprotein (cyt c and BOD) multilayer systems are studied by SPR and cyclic voltammetry. For the layer construction, sulfonated polyaniline is used. The only cyt c containing multilayer electrodes show that the quantity of deposited protein and the kinetic behavior depend on the cyt c form incorporated. In the case of the biprotein multilayer with BOD, it is demonstrated that the catalytic signal chain from the electrode via cyt c to BOD and oxygen can be established with all chosen cyt c forms. However, the magnitude of the catalytic current as well as the kinetic behavior differ significantly. We conclude that the different cytochrome c forms affect three parameters, identified here, to be important for the functionality of the multilayer system: the amount of molecules per layer, which can be immobilized on the electrodes, the cyt c self-exchange rate, and the rate constant for the reaction with BOD
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cytochromes c
|2 NLM
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|a 9007-43-6
|2 NLM
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|a Oxidoreductases Acting on CH-CH Group Donors
|2 NLM
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|a EC 1.3.-
|2 NLM
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|a bilirubin oxidase
|2 NLM
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|a EC 1.3.3.5
|2 NLM
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|a Turano, Paola
|e verfasserin
|4 aut
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|a Allegrozzi, Marco
|e verfasserin
|4 aut
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|a Möhwald, Helmuth
|e verfasserin
|4 aut
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| 700 |
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|a Lisdat, Fred
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 7 vom: 05. Apr., Seite 4202-11
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:27
|g year:2011
|g number:7
|g day:05
|g month:04
|g pages:4202-11
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|u http://dx.doi.org/10.1021/la104964z
|3 Volltext
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