A stress-associated protein containing A20/AN1 zing-finger domains expressed in Medicago truncatula seeds

Copyright © 2011 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 49(2011), 3 vom: 01. März, Seite 303-10
Auteur principal: Gimeno-Gilles, Christine (Auteur)
Autres auteurs: Gervais, Marie-Laure, Planchet, Elisabeth, Satour, Pascale, Limami, Anis M, Lelievre, Eric
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Research Support, Non-U.S. Gov't Basic-Leucine Zipper Transcription Factors Carrier Proteins Globulins Plant Proteins Seed Storage Proteins Abscisic Acid 72S9A8J5GW vicilin protein, plant plus... 9067-60-1 Oxygen S88TT14065
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100 1 |a Gimeno-Gilles, Christine  |e verfasserin  |4 aut 
245 1 2 |a A stress-associated protein containing A20/AN1 zing-finger domains expressed in Medicago truncatula seeds 
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520 |a Copyright © 2011 Elsevier Masson SAS. All rights reserved. 
520 |a MtSAP1 (Medicago truncatula stress-associated protein 1) was revealed as a down-regulated gene by suppressive subtractive hybridization between two mRNA populations of embryo axes harvested before and after radicle emergence. MtSAP1 is the first gene encoding a SAP with A20 and AN1 zinc-finger domains characterized in M. truncatula. MtSAP1 protein shares 54% and 62% homology with AtSAP7 (Arabidopsis thaliana) and OsiSAP8 (Oryza sativa) respectively, with in particular a strong homology in the A20 and AN1 conserved domains. MtSAP1 gene expression increased in the embryos during the acquisition of tolerance to desiccation, reached its maximum in dry seed and decreased dramatically during the first hours of imbibition. Abiotic stresses (cold and hypoxia), abscisic acid and desiccation treatments induced MtSAP1 gene expression and protein accumulation in embryo axis, while mild drought stress did not affect significantly its expression. This profile of expression along with the presence of anaerobic response elements and ABRE sequences in the upstream region of the gene is consistent with a role of MtSAP1 in the tolerance of low oxygen availability and desiccation during late stages of seed maturation. Silencing of MtSAP1 by RNA interference (RNAi) showed that the function of the encoded protein is required for adequate accumulation of storage globulin proteins, vicilin and legumin, and for the development of embryos able to achieve successful germination 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Basic-Leucine Zipper Transcription Factors  |2 NLM 
650 7 |a Carrier Proteins  |2 NLM 
650 7 |a Globulins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Seed Storage Proteins  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a vicilin protein, plant  |2 NLM 
650 7 |a 9067-60-1  |2 NLM 
650 7 |a Oxygen  |2 NLM 
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700 1 |a Gervais, Marie-Laure  |e verfasserin  |4 aut 
700 1 |a Planchet, Elisabeth  |e verfasserin  |4 aut 
700 1 |a Satour, Pascale  |e verfasserin  |4 aut 
700 1 |a Limami, Anis M  |e verfasserin  |4 aut 
700 1 |a Lelievre, Eric  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 49(2011), 3 vom: 01. März, Seite 303-10  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:49  |g year:2011  |g number:3  |g day:01  |g month:03  |g pages:303-10 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2011.01.004  |3 Volltext 
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