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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2011.01.007
|2 doi
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|a pubmed25n0685.xml
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|a (NLM)21295988
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Tiwari, Krishnaraj
|e verfasserin
|4 aut
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|a Microarray analysis of ripening-regulated gene expression and its modulation by 1-MCP and hexanal
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.10.2011
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Masson SAS. All rights reserved.
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|a Hexanal, an inhibitor of phospholipase D, has been successfully applied for the pre- and post-harvest treatment of fruits, vegetables and flowers. Changes in gene expression induced by hexanal and the ethylene antagonist 1-MCP, were analyzed by microarray using TOM2 tomato oligo-array containing approximately 12 000 unigenes. Mature green tomato fruits were treated with 1-MCP and hexanal, RNA isolated after 10 days of storage, and labeled cDNA synthesized for microarray analysis. A large variation in gene expression profile was observed in 1-MCP-treated fruits. Genes for ethylene biosynthetic pathway enzymes such as ACC- synthase/oxidase, ethylene receptor and ethylene response factors were heavily down-regulated in 1-MCP-treated fruits. In addition, genes for key enzymes involved in cell wall degradation and carotenoid development pathways were down-regulated. Hexanal treatment significantly down-regulated ACC-synthase, and to a lesser extent, other components of ethylene signal transduction. By contrast to MCP-treated fruits, hexanal-treated fruits gradually ripened and showed higher levels of lycopene and β-carotene. GC-MS analysis of volatiles showed a higher level of major volatile components in hexanal-treated fruits. Similarities in the modulation of gene expression by hexanal and 1-MCP suggest that hexanal, in addition to being a PLD inhibitor, may also act as a weak ethylene inhibitor
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aldehydes
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|a DNA, Complementary
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|a Enzymes
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|a Ethylenes
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|a Volatile Organic Compounds
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|a Carotenoids
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|a 36-88-4
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|a RNA
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|a 63231-63-0
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|a ethylene
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|a 91GW059KN7
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|a n-hexanal
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|a 9DC2K31JJQ
|2 NLM
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|a Phospholipase D
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|a EC 3.1.4.4
|2 NLM
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|a Lyases
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|a EC 4.-
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|a 1-aminocyclopropanecarboxylate synthase
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|a EC 4.4.1.14
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|a Lycopene
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|a SB0N2N0WV6
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|a Paliyath, Gopinadhan
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 49(2011), 3 vom: 01. März, Seite 329-40
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:49
|g year:2011
|g number:3
|g day:01
|g month:03
|g pages:329-40
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|u http://dx.doi.org/10.1016/j.plaphy.2011.01.007
|3 Volltext
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