Microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of PCR-amplified genes

Spent sulfidic caustic (SSC) produced from petrochemical plants contains a high concentration of hydrogen sulfide and alkalinity, and some almost non-biodegradable organic compounds such as benzene, toluene, ethylbenzene and xylenes (BTEX). SSC is mainly incinerated with auxiliary fuel, leading to s...

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Veröffentlicht in:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 63(2011), 3 vom: 01., Seite 475-83
1. Verfasser: Lee, J-H (VerfasserIn)
Weitere Verfasser: Lee, S-M, Choi, G-C, Park, H-S, Kang, D-H, Park, J-J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Water science and technology : a journal of the International Association on Water Pollution Research
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Caustics DNA, Ribosomal Quaternary Ammonium Compounds Sulfides Volatile Organic Compounds Nitrogen N762921K75
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245 1 0 |a Microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of PCR-amplified genes 
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500 |a Date Revised 21.11.2013 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a Spent sulfidic caustic (SSC) produced from petrochemical plants contains a high concentration of hydrogen sulfide and alkalinity, and some almost non-biodegradable organic compounds such as benzene, toluene, ethylbenzene and xylenes (BTEX). SSC is mainly incinerated with auxiliary fuel, leading to secondary pollution problems. The reuse of this waste is becoming increasingly important from economic and environmental viewpoints. To denitrify wastewater with low COD/N ratio, additional carbon sources are required. Thus, autotrophic denitrification has attracted increasing attention. In this study, SSC was injected as an electron donor for sulfur-based autotrophic denitrification in the modified Ludzack-Ettinger (MLE) process. The efficiencies of nitrification, COD, and total nitrogen (TN) removal were evaluated with varying SSC dosage. Adequate SSC injection exhibited stable autotrophic denitrification. No BTEX were detected in the monitored BTEX concentrations of the effluent. To analyse the microbial community of the MLE process, PCR-DGGE based on 16 S rDNA with EUB primers, TD primers and nirK gene with nirK primers was performed in order to elucidate the application of the MLE process to SSC 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Caustics  |2 NLM 
650 7 |a DNA, Ribosomal  |2 NLM 
650 7 |a Quaternary Ammonium Compounds  |2 NLM 
650 7 |a Sulfides  |2 NLM 
650 7 |a Volatile Organic Compounds  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
700 1 |a Lee, S-M  |e verfasserin  |4 aut 
700 1 |a Choi, G-C  |e verfasserin  |4 aut 
700 1 |a Park, H-S  |e verfasserin  |4 aut 
700 1 |a Kang, D-H  |e verfasserin  |4 aut 
700 1 |a Park, J-J  |e verfasserin  |4 aut 
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773 1 8 |g volume:63  |g year:2011  |g number:3  |g day:01  |g pages:475-83 
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