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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2010.12.006
|2 doi
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|a pubmed24n0684.xml
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|a (DE-627)NLM205257186
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|a (NLM)21256620
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Jayakannan, Maheswari
|e verfasserin
|4 aut
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|a Improved measurements of Na+ fluxes in plants using calixarene-based microelectrodes
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.09.2012
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.
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|a Ion-selective microelectrodes are a powerful tool in studying adaptive responses of plant cells and tissues to various abiotic stresses. However, application of this technique in Na(+) flux measurements was limited due to poor selectivity for Na(+) ions of commercially available Na(+) cocktails. Often, these cocktails cannot discriminate between Na(+) and other interfering ions such as K(+) and Ca(2+), leading to inaccurate measurements of Na(+) concentration and, consequently, inaccurate Na(+) flux calculations. To overcome this problem, three Na(+)-selective cocktail mixtures were prepared using tetramethoxyethyl ester derivative of p-t-butyl calix[4]arene. These cocktail mixtures were compared with commercially available ETH 227-based Na(+) cocktail for selectivity for Na(+) ions over other ions (particularly K(+) and Ca(2+)). Among the three calixarene-based Na(+) cocktails tested, cocktail 2 [in % w/w: Na(+) ionophore (4-tert-butylcalix[4]arene-tetra acetic acid tetraethyl ester) 3.5, the plasticizer (2-nitrophenyl octyl ether) 95.9 and lipophilic anion (potassium tetrakis (4-chlorophenyl) borate) 0.6] showed the best selectivity for Na(+) ions over K(+) and Ca(2+) ions and was highly stable over time (up to 10h). Na(+) flux measurements under a wide range of NaCl concentrations (25-150 mM) using Na(+) cocktail 2 established a clear dose-response relationship between severity of salt stress and magnitude of Na(+) influx at the distal elongation and mature zones of Arabidopsis thaliana roots. Furthermore, Na(+) cocktail 2 was compared with commercially available ETH 227-based Na(+) cocktail by measuring Na(+) fluxes at the two Arabidopsis root zones in response to 100mM NaCl treatment. With calixarene-based Na(+) cocktail 2, a large decreasing Na(+) influx (0-15 min) followed by small Na(+) influx (15-45 min) was measured, whereas with ETH-based Na(+) cocktail Na(+) influx was short-lived (1-3 min) and was followed by Na(+) efflux (3-45 min) that might have been due to K(+) and Ca(2+) efflux measured together with Na(+) influx. In conclusion, Na(+)-selective calixarene-based microelectrodes have excellent potential to be used in real-time Na(+) flux measurements in plants
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acetamides
|2 NLM
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|a Ionophores
|2 NLM
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|a Calixarenes
|2 NLM
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|a 130036-26-9
|2 NLM
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|a Sodium Chloride
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|a 451W47IQ8X
|2 NLM
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|a ETH 227
|2 NLM
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|a 61183-76-4
|2 NLM
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|a Sodium
|2 NLM
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|a 9NEZ333N27
|2 NLM
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|a Potassium
|2 NLM
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|a RWP5GA015D
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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1 |
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|a Babourina, Olga
|e verfasserin
|4 aut
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|a Rengel, Zed
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 10 vom: 01. Juli, Seite 1045-51
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:10
|g day:01
|g month:07
|g pages:1045-51
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|u http://dx.doi.org/10.1016/j.jplph.2010.12.006
|3 Volltext
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|d 168
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|h 1045-51
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