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|a pubmed25n0684.xml
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|a (DE-627)NLM205207219
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|a (NLM)21251364
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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|a Li, Jia-huan
|e verfasserin
|4 aut
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|a Effect of sulfonic tanshinone sodium injection on the expression and activity of aquaporin-5 of human alveolar epithelial cells after seawater exposure
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 30.01.2012
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|a Date Revised 10.12.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To explore the effects of tanshinone IIA on the activity of aquaporin-5 (AQP5) in human alveolar epithelial cells (A549) after seawater exposure and its possible mechanism
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|a METHODS: Routinely cultured A549 cells were divided into different groups according to different content of seawater: blank control group, 15%, 25%, 50%, 75%, 100% seawater groups; they were divided into different groups according to the duration of exposure to 25% seawater: blank control group, 1, 4, 8 hours groups; they were also divided into different groups according to concentration of tanshinone IIA and exposed to seawater for 4 hours: blank control group, 25% seawater group, 25, 50, 75, 100 μg/ml tanshinone IIA intervention groups. The expressions of AQP5 were respectively assayed by Western blotting and immunohistochemistry
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|a RESULTS: The results of Western blotting showed that the expressions of AQP5 were remarkably higher at 8 hours of exposure to seawater in 25% and 50% seawater groups than those in blank control group (1.053±0.231, 1.116±0.316 vs. 0.101±0.081, both P<0.05); the expression of AQP5 in 1-hour group showed a slight increase compared with blank control group (0.306±0.125 vs. 0.288±0.098, P>0.05), that in 4-hour group was increased significantly (1.423±0.377, P<0.01), and in 8-hour group (1.507±0.461) it was slightly higher than that in 4-hour group without statistical significance. The AQP5 expression was significantly lower in tanshinone IIA 25 μg/ml and 50 μg/ml intervention groups than that in 25% seawater group (0.580±0.186, 0.499±0.172 vs. 1.013±0.287, both P<0.05). Immuno-histochemistry showed that the expression of AQP5 was markedly up-regulated after A549 cells were stimulated with 25% seawater for 4 hours as compared with blank control group (7.21±0.78 vs. 0.41±0.07, P<0.01), but intervention of tanshinone IIA significantly inhibited the up-regulation of AQP5 expression (3.02±0.23) induced by 25% seawater (P<0.05)
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|a CONCLUSION: The experimental results showed that tanshinone IIA is innocuous to A549 at a dosage of 25 μg/ml, and it can decrease the overexpression of AQP5 induced by seawater
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a AQP5 protein, human
|2 NLM
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| 650 |
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|a Abietanes
|2 NLM
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| 650 |
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|a Aquaporin 5
|2 NLM
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| 650 |
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|a tanshinone
|2 NLM
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| 650 |
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|a 03UUH3J385
|2 NLM
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| 700 |
1 |
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|a Xu, Min
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fan, Qi-xin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Xiao-yan
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Yong
|e verfasserin
|4 aut
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| 700 |
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|a Zhao, Peng-tao
|e verfasserin
|4 aut
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|a Zhang, Bo
|e verfasserin
|4 aut
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|a Li, Zhi-chao
|e verfasserin
|4 aut
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| 700 |
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|a Jin, Fa-guang
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 23(2011), 1 vom: 20. Jan., Seite 32-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:23
|g year:2011
|g number:1
|g day:20
|g month:01
|g pages:32-5
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 23
|j 2011
|e 1
|b 20
|c 01
|h 32-5
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