AtCPL5, a novel Ser-2-specific RNA polymerase II C-terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis

AtCPL5, a novel Ser-2-specific RNA polymerase II C-terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis

© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Détails bibliographiques
Publié dans:The New phytologist. - 1990. - 190(2011), 1 vom: 01. Apr., Seite 57-74
Auteur principal: Jin, Yong-Mei (Auteur)
Autres auteurs: Jung, Jinwook, Jeon, Hyesung, Won, So Youn, Feng, Yue, Kang, Jae-Sook, Lee, Sang Yeol, Cheong, Jong-Joo, Koiwa, Hisashi, Kim, Minkyun
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. ABA Arabidopsis CPL RNAPII CTD phosphatase drought stress Arabidopsis Proteins DNA, Complementary plus... RNA, Messenger Phosphoserine 17885-08-4 Abscisic Acid 72S9A8J5GW RNA Polymerase II EC 2.7.7.- Glucuronidase EC 3.2.1.31
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520 |a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust. 
520 |a Arabidopsis RNA polymerase II (RNAPII) C-terminal domain (CTD) phosphatases regulate stress-responsive gene expression and plant development via the dephosphorylation of serine (Ser) residues of the CTD. Some of these phosphatases (CTD phosphatase-like 1 (CPL1) to CPL3) negatively regulate ABA and stress responses. Here, we isolated AtCPL5, a cDNA encoding a protein containing two CTD phosphatase domains (CPDs). To characterize AtCPL5, we analyzed the gene expression patterns and subcellular protein localization, investigated various phenotypes of AtCPL5-overexpressors and knockout mutants involved in ABA and drought responses, performed microarray and RNA hybridization analyses using AtCPL5-overexpressors, and assessed the CTD phosphatase activities of the purified AtCPL5 and each CPD of the protein. Transcripts of the nucleus-localized AtCPL5 were induced by ABA and drought. AtCPL5-overexpressors exhibited ABA-hypersensitive phenotypes (increased inhibition of seed germination, seedling growth, and stomatal aperture), lower transpiration rates upon dehydration, and enhanced drought tolerance, while the knockout mutants showed weak ABA hyposensitivity. AtCPL5 overexpression changed the expression of numerous genes, including those involved in ABA-mediated responses. In contrast to Ser-5-specific phosphatase activity of the negative stress response regulators, purified AtCPL5 and each CPD of the protein specifically dephosphorylated Ser-2 in RNAPII CTD. We conclude that AtCPL5 is a unique CPL family protein that positively regulates ABA-mediated development and drought responses in Arabidopsis 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a ABA 
650 4 |a Arabidopsis 
650 4 |a CPL 
650 4 |a RNAPII CTD phosphatase 
650 4 |a drought stress 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a Phosphoserine  |2 NLM 
650 7 |a 17885-08-4  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a RNA Polymerase II  |2 NLM 
650 7 |a EC 2.7.7.-  |2 NLM 
650 7 |a Glucuronidase  |2 NLM 
650 7 |a EC 3.2.1.31  |2 NLM 
700 1 |a Jung, Jinwook  |e verfasserin  |4 aut 
700 1 |a Jeon, Hyesung  |e verfasserin  |4 aut 
700 1 |a Won, So Youn  |e verfasserin  |4 aut 
700 1 |a Feng, Yue  |e verfasserin  |4 aut 
700 1 |a Kang, Jae-Sook  |e verfasserin  |4 aut 
700 1 |a Lee, Sang Yeol  |e verfasserin  |4 aut 
700 1 |a Cheong, Jong-Joo  |e verfasserin  |4 aut 
700 1 |a Koiwa, Hisashi  |e verfasserin  |4 aut 
700 1 |a Kim, Minkyun  |e verfasserin  |4 aut 
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