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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la1025944
|2 doi
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|a pubmed24n1328.xml
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|a (DE-627)NLM20497755X
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|a (NLM)21226498
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Heitz, Benjamin A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Polymerized planar suspended lipid bilayers for single ion channel recordings
|b comparison of several dienoyl lipids
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.06.2011
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|a Date Revised 14.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The stabilization of suspended planar lipid membranes, or black lipid membranes (BLMs), through polymerization of mono- and bis-functionalized dienoyl lipids was investigated. Electrical properties, including capacitance, conductance, and dielectric breakdown voltage, were determined for BLMs composed of mono-DenPC, bis-DenPC, mono-SorbPC, and bis-SorbPC both prior to and following photopolymerization, with diphytanoyl phosphocholine (DPhPC) serving as a control. Poly(lipid) BLMs exhibited significantly longer lifetimes and increased the stability of air-water transfers. BLM stability followed the order bis-DenPC > mono-DenPC ≈ mono-SorbPC > bis-SorbPC. The conductance of bis-SorbPC BLMs was significantly higher than that of the other lipids, which is attributed to a high density of hydrophilic pores, resulting in relatively unstable membranes. The use of poly(lipid) BLMs as matrices for supporting the activity of an ion channel protein (IC) was explored using α-hemolysin (α-HL), a model IC. Characteristic i-V plots of α-HL were maintained following photopolymerization of bis-DenPC, mono-DenPC, and mono-SorbPC, demonstrating the utility of these materials for preparing more durable BLMs for single-channel recordings of reconstituted ICs
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|a Comparative Study
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Hemolysin Proteins
|2 NLM
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|a Ion Channels
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Suspensions
|2 NLM
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| 650 |
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|a Phosphorylcholine
|2 NLM
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| 650 |
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|a 107-73-3
|2 NLM
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| 700 |
1 |
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|a Xu, Juhua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jones, Ian W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Keogh, John P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Comi, Troy J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hall, Henry K
|c Jr
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aspinwall, Craig A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Saavedra, S Scott
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 5 vom: 01. März, Seite 1882-90
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:27
|g year:2011
|g number:5
|g day:01
|g month:03
|g pages:1882-90
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| 856 |
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|u http://dx.doi.org/10.1021/la1025944
|3 Volltext
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