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|a 10.1111/j.1469-8137.2010.03597.x
|2 doi
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|a eng
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| 100 |
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|a Tisserant, E
|e verfasserin
|4 aut
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| 245 |
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|a Deep RNA sequencing improved the structural annotation of the Tuber melanosporum transcriptome
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 09.08.2011
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|a Date Revised 14.04.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.
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|a • The functional complexity of the Tuber melanosporum transcriptome has not yet been fully elucidated. Here, we applied high-throughput Illumina RNA-sequencing (RNA-Seq) to the transcriptome of T. melanosporum at different major developmental stages, that is free-living mycelium, fruiting body and ectomycorrhiza. • Sequencing of cDNA libraries generated a total of c. 24 million sequence reads representing > 882 Mb of sequence data. To construct a coverage signal profile across the genome, all reads were then aligned to the reference genome assembly of T. melanosporum Mel28. • We were able to identify a substantial number of novel transcripts, antisense transcripts, new exons, untranslated regions (UTRs), alternative upstream initiation codons and upstream open reading frames. • This RNA-Seq analysis allowed us to improve the genome annotation. It also provided us with a genome-wide view of the transcriptional and post-transcriptional mechanisms generating an increased number of transcript isoforms during major developmental transitions in T. melanosporum
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Codon, Initiator
|2 NLM
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| 650 |
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|a RNA, Fungal
|2 NLM
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| 650 |
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|a Untranslated Regions
|2 NLM
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| 700 |
1 |
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|a Da Silva, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kohler, A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Morin, E
|e verfasserin
|4 aut
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| 700 |
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|a Wincker, P
|e verfasserin
|4 aut
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|a Martin, F
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 189(2011), 3 vom: 15. Feb., Seite 883-891
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:189
|g year:2011
|g number:3
|g day:15
|g month:02
|g pages:883-891
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|u http://dx.doi.org/10.1111/j.1469-8137.2010.03597.x
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