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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la104346k
|2 doi
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|a DE-627
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|a eng
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|a Tian, Feng
|e verfasserin
|4 aut
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|a Peptide separation through a CB[8]-mediated supramolecular trap-and-release process
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 26.05.2011
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We demonstrate a supramolecular peptide separation approach by the selective immobilization of peptides bearing an N-terminal tryptophan onto a CB[8]-modified gold substrate, followed by electrochemical release. The CB[8]-stabilized heteroternary complexes were characterized by (1)H NMR, ESI-MS, UV/vis, and fluorescence spectroscopy and cyclic voltammetry. Micropatterned CB[8]-modified gold substrates were found to trap only the recognizable N-tryptophan-containing peptides from a peptide mixture that could be visualized as green peptide arrays under fluorescence microscopy. Subsequently, the bound peptides were released from the modified substrates by the controlled single-electron reduction of viologen. The fully reversible trap-and-release process was repeated for 13 cycles, and the cumulative release profile of the dye-peptide conjugate was monitored by fluorescence spectroscopy, indicating that no degradation occurred
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Peptides
|2 NLM
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|a Tryptophan
|2 NLM
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|a 8DUH1N11BX
|2 NLM
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|a Cziferszky, Monika
|e verfasserin
|4 aut
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|a Jiao, Dezhi
|e verfasserin
|4 aut
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|a Wahlström, Karolina
|e verfasserin
|4 aut
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|a Geng, Jin
|e verfasserin
|4 aut
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|a Scherman, Oren A
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 4 vom: 15. Feb., Seite 1387-90
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:27
|g year:2011
|g number:4
|g day:15
|g month:02
|g pages:1387-90
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|u http://dx.doi.org/10.1021/la104346k
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