Peptide separation through a CB[8]-mediated supramolecular trap-and-release process

We demonstrate a supramolecular peptide separation approach by the selective immobilization of peptides bearing an N-terminal tryptophan onto a CB[8]-modified gold substrate, followed by electrochemical release. The CB[8]-stabilized heteroternary complexes were characterized by (1)H NMR, ESI-MS, UV/...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 27(2011), 4 vom: 15. Feb., Seite 1387-90
1. Verfasser: Tian, Feng (VerfasserIn)
Weitere Verfasser: Cziferszky, Monika, Jiao, Dezhi, Wahlström, Karolina, Geng, Jin, Scherman, Oren A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Peptides Tryptophan 8DUH1N11BX
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245 1 0 |a Peptide separation through a CB[8]-mediated supramolecular trap-and-release process 
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520 |a We demonstrate a supramolecular peptide separation approach by the selective immobilization of peptides bearing an N-terminal tryptophan onto a CB[8]-modified gold substrate, followed by electrochemical release. The CB[8]-stabilized heteroternary complexes were characterized by (1)H NMR, ESI-MS, UV/vis, and fluorescence spectroscopy and cyclic voltammetry. Micropatterned CB[8]-modified gold substrates were found to trap only the recognizable N-tryptophan-containing peptides from a peptide mixture that could be visualized as green peptide arrays under fluorescence microscopy. Subsequently, the bound peptides were released from the modified substrates by the controlled single-electron reduction of viologen. The fully reversible trap-and-release process was repeated for 13 cycles, and the cumulative release profile of the dye-peptide conjugate was monitored by fluorescence spectroscopy, indicating that no degradation occurred 
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700 1 |a Cziferszky, Monika  |e verfasserin  |4 aut 
700 1 |a Jiao, Dezhi  |e verfasserin  |4 aut 
700 1 |a Wahlström, Karolina  |e verfasserin  |4 aut 
700 1 |a Geng, Jin  |e verfasserin  |4 aut 
700 1 |a Scherman, Oren A  |e verfasserin  |4 aut 
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