|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM204653231 |
| 003 |
DE-627 |
| 005 |
20250212081224.0 |
| 007 |
tu |
| 008 |
231223s2010 xx ||||| 00| ||chi c |
| 028 |
5 |
2 |
|a pubmed25n0682.xml
|
| 035 |
|
|
|a (DE-627)NLM204653231
|
| 035 |
|
|
|a (NLM)21190604
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a chi
|
| 100 |
1 |
|
|a Liu, Qing-juan
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The effect of Janus kinase 2 inhibitor AG490 on renal tubular epithelial-myofibroblast transdifferentiation induced by interleukin-1β
|
| 264 |
|
1 |
|c 2010
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 23.01.2012
|
| 500 |
|
|
|a Date Revised 15.11.2012
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a OBJECTIVE: To investigate the effect of AG490, a Janus kinase 2 inhibitor, on epithelial-myofibroblast transdifferentiation induced by interleukin-1β (IL-1β)
|
| 520 |
|
|
|a METHODS: Cultured human renal tubular epithelial cell line (HKCs) were divided into three groups: blank control group, IL-1β (5 ng/ml) group and AG490 group (IL-1β 5 ng/ml+AG490 10 μmol/L). The cells in all groups were collected at 24, 48, 72 hours after intervention. Immunocytochemistry and Western blotting analysis were used to determine the expressions of cytokeratin-18 (CK-18) and α-smooth muscle actin (α-SMA)
|
| 520 |
|
|
|a RESULTS: The higher expression of CK-18 (1.25±0.08) and mild expression of α-SMA (0.17±0.01) were found in blank control group. In IL-1β group, the protein level of CK-18 was gradually decreased with prolongation of stimulus (24 hours : 0.69±0.04, 48 hours: 0.52±0.03, 72 hours: 0.30±0.01), while the expression level of α-SMA was gradually increased (24 hours: 0.56±0.04, 48 hours: 1.05±0.07, 72 hours: 1.43±0.07), and the difference between blank control group and IL-1β group was statistically significant (all P<0.05). The administration of AG490 could restore the expression of CK-18 (24 hours: 1.07±0.07, 48 hours: 0.93±0.06, 72 hours: 0.83±0.06), and inhibit the expression of α-SMA induced by IL-1β (24 hours: 0.33± 0.01, 48 hours: 0.52±0.01, 72 hours: 0.61±0.04). There was significant difference between AG490 group and IL-1β group (all P<0.05). The results of immunocytochemistry and that of Western blotting were identical
|
| 520 |
|
|
|a CONCLUSION: IL-1β can induce the transdifferentiation of renal tubular epithelial cells, up-regulate the expression of α-SMA, induce the renal tubular epithelial cells to transform to myofibroblast, while AG490 can inhibit the effect of IL-1β
|
| 650 |
|
4 |
|a English Abstract
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a ACTA2 protein, human
|2 NLM
|
| 650 |
|
7 |
|a Actins
|2 NLM
|
| 650 |
|
7 |
|a Enzyme Inhibitors
|2 NLM
|
| 650 |
|
7 |
|a Interleukin-1beta
|2 NLM
|
| 650 |
|
7 |
|a Keratin-18
|2 NLM
|
| 650 |
|
7 |
|a Tyrphostins
|2 NLM
|
| 650 |
|
7 |
|a alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide
|2 NLM
|
| 650 |
|
7 |
|a Janus Kinase 2
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.10.2
|2 NLM
|
| 700 |
1 |
|
|a Shi, Yong-hong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liu, Shu-xia
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hao, Jun
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cao, Yan-ping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Hang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wei, Jin-ying
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Duan, Hui-jun
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 22(2010), 12 vom: 30. Dez., Seite 747-9
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
|
| 773 |
1 |
8 |
|g volume:22
|g year:2010
|g number:12
|g day:30
|g month:12
|g pages:747-9
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 22
|j 2010
|e 12
|b 30
|c 12
|h 747-9
|