Optimization of salt concentration in PEG-based crystallization solutions

Although polyethylene glycol (PEG) is the most widely used precipitant in protein crystallization, the concentration of co-existing salt in the solution has not been well discussed. To determine the optimum salt concentration range, several kinds of protein were crystallized in a 30% PEG 4000 soluti...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 18(2011), 1 vom: 28. Jan., Seite 84-7
1. Verfasser: Yamanaka, Mari (VerfasserIn)
Weitere Verfasser: Inaka, Koji, Furubayashi, Naoki, Matsushima, Masaaki, Takahashi, Sachiko, Tanaka, Hiroaki, Sano, Satoshi, Sato, Masaru, Kobayashi, Tomoyuki, Tanaka, Tetsuo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Solutions Polyethylene Glycols 3WJQ0SDW1A Sodium Chloride 451W47IQ8X alpha-Amylases EC 3.2.1.1 Muramidase mehr... EC 3.2.1.17 Aldose-Ketose Isomerases EC 5.3.1.- xylose isomerase EC 5.3.1.5