Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation
Detection of protein-protein interactions in cells is crucial for understanding the biological functions of proteins, including their roles in signal transduction. However, current methods require specific antibodies both for immunoprecipitation and detection, making them expensive and sometimes unr...
| Publié dans: | BioTechniques. - 1991. - 49(2010), 6 vom: 01. Dez., Seite 881-6 |
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| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2010
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, N.I.H., Extramural Escherichia coli Proteins Immunoglobulin G Recombinant Fusion Proteins Repressor Proteins enhanced green fluorescent protein Green Fluorescent Proteins 147336-22-9 Glutathione Transferase plus... |
| Accès en ligne |
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