Enzyme:nanoparticle bioconjugates with two sequential enzymes : stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles
We report the synthesis and characterization of bioconjugates in which the enzymes malate dehydrogenase (MDH) and/or citrate synthase (CS) were adsorbed to 30 nm diameter Au nanoparticles. Enzyme:Au stoichiometry and kinetic parameters (specific activity, k(cat), K(M), and activity per particle) wer...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 24 vom: 21. Dez., Seite 18992-9000 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Fluorescent Dyes Gold 7440-57-5 Malate Dehydrogenase EC 1.1.1.37 Citrate (si)-Synthase EC 2.3.3.1 |
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