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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2010.10.003
|2 doi
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|a pubmed24n0680.xml
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|a (NLM)21106274
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|a DE-627
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|e rakwb
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|a eng
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|a Himuro, Yasuyo
|e verfasserin
|4 aut
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|a FOX-superroots of Lotus corniculatus, overexpressing Arabidopsis full-length cDNA, show stable variations in morphological traits
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|c 2011
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.04.2011
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2010 Elsevier GmbH. All rights reserved.
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|a Using the full-length cDNA overexpressor (FOX) gene-hunting system, we have generated 130 Arabidopsis FOX-superroot lines in bird's-foot trefoil (Lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. We used the Arabidopsis-FOX Agrobacterium library (constructed by ligating pBIG2113SF) for the Agrobacterium-mediated transformation of superroots (SR) and the subsequent selection of gain-of-function mutants with ectopically expressed Arabidopsis genes. The original superroot culture of L. corniculatus is a unique host system displaying fast root growth in vitro, allowing continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely hormone-free culture conditions. Several of the Arabidopsis FOX-superroot lines show interesting deviations from normal growth and morphology of roots from SR-plants, such as differences in pigmentation, growth rate, length or diameter. Some of these mutations are of potential agricultural interest. Genomic PCR analysis revealed that 100 (76.9%) out of the 130 transgenic lines showed the amplification of single fragments. Sequence analysis of the PCR fragments from these 100 lines identified full-length cDNA in 74 of them. Forty-three out of 74 full-length cDNA carried known genes. The Arabidopsis FOX-superroot lines of L. corniculatus, produced in this study, expand the FOX hunting system and provide a new tool for the genetic analysis and control of root growth in a leguminous forage plant
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|a Journal Article
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|a DNA, Complementary
|2 NLM
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|a Tanaka, Hidenori
|e verfasserin
|4 aut
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|a Hashiguchi, Masatsugu
|e verfasserin
|4 aut
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|a Ichikawa, Takanari
|e verfasserin
|4 aut
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|a Nakazawa, Miki
|e verfasserin
|4 aut
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|a Seki, Motoaki
|e verfasserin
|4 aut
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|a Fujita, Miki
|e verfasserin
|4 aut
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|a Shinozaki, Kazuo
|e verfasserin
|4 aut
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|a Matsui, Minami
|e verfasserin
|4 aut
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|a Akashi, Ryo
|e verfasserin
|4 aut
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|a Hoffmann, Franz
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 2 vom: 15. Jan., Seite 181-7
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|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:2
|g day:15
|g month:01
|g pages:181-7
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|u http://dx.doi.org/10.1016/j.jplph.2010.10.003
|3 Volltext
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