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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2010.03556.x
|2 doi
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|a pubmed25n0679.xml
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|a DE-627
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|a eng
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|a Chen, Aiqun
|e verfasserin
|4 aut
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|a Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 18.05.2011
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.
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|a • In this study, six putative promoter regions of phosphate transporter Pht1;3, Pht1;4 and Pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1;3 and Pht1;4/Pht1;5. • Histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). • A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements--the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS--mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. • Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Phosphate Transport Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Glucuronidase
|2 NLM
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|a EC 3.2.1.31
|2 NLM
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|a Gu, Mian
|e verfasserin
|4 aut
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|a Sun, Shubin
|e verfasserin
|4 aut
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|a Zhu, Lingling
|e verfasserin
|4 aut
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|a Hong, Shuai
|e verfasserin
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|a Xu, Guohua
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 189(2011), 4 vom: 09. März, Seite 1157-1169
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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|g volume:189
|g year:2011
|g number:4
|g day:09
|g month:03
|g pages:1157-1169
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|u http://dx.doi.org/10.1111/j.1469-8137.2010.03556.x
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