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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2010.10.009
|2 doi
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|a pubmed25n0679.xml
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|a (NLM)21093280
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|a DE-627
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|e rakwb
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|a eng
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|a Rosales, Eliana Paola
|e verfasserin
|4 aut
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|a Nitric oxide inhibits nitrate reductase activity in wheat leaves
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.05.2011
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2010 Elsevier Masson SAS. All rights reserved.
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|a Nitrate reductase (NR), a committed enzyme in nitrate assimilation, is involved in the generation of nitric oxide (NO) in plants. In wheat leaf segments exposed to sodium nitroprusside (SNP) or S-nitrosoglutathione (GSNO), NR activity was significantly reduced to different degrees between 3 and 21 h, whereas its activity was partially recovered when the NO scavenger cPTIO was used. At 21 h, NR activity decreased from 38% with 10 μM SNP to 91% with 500 μM SNP, respect to the C values. S-nitrosoglutathione reduced NR activity between 18% and 26% only at 3 h. When added directly to the incubation solution, NR activity was quickly and strongly inhibited more than 90% by 10 or 50 μM SNP, whereas 10 μM GSNO reduced the enzyme activity an average of 50%, at 30 min of incubation. l-NAME and d-arginine (nitric oxide synthase (NOS) inhibitors) increased NR activity by 14% and 52% respectively, at 21 h of exposure, leading us to suppose that endogenous NOS-dependent NO formation could also be modulating NR activity. NR protein expression was not affected by 10 or 100 μM SNP at 3 or 21 h of incubation, whereas nitration of tyrosines was not detected in the NR protein. Nitrates, which content increased along the time in the tissues, could be exerting a role in this regulation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Nitrates
|2 NLM
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|a Nitroprusside
|2 NLM
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|a 169D1260KM
|2 NLM
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|a Nitric Oxide
|2 NLM
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|a 31C4KY9ESH
|2 NLM
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|a S-Nitrosoglutathione
|2 NLM
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|a 57564-91-7
|2 NLM
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|a Nitrate Reductase
|2 NLM
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| 650 |
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|a EC 1.7.99.4
|2 NLM
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1 |
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|a Iannone, María Florencia
|e verfasserin
|4 aut
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1 |
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|a Groppa, María Daniela
|e verfasserin
|4 aut
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| 700 |
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|a Benavides, María Patricia
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 49(2011), 2 vom: 01. Feb., Seite 124-30
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:49
|g year:2011
|g number:2
|g day:01
|g month:02
|g pages:124-30
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|u http://dx.doi.org/10.1016/j.plaphy.2010.10.009
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