A ligation-independent cloning method using nicking DNA endonuclease
Using nicking DNA endonuclease (NiDE), we developed a novel technique to clone DNA fragments into plasmids. We created a NiDE cassette consisting of two inverted NiDE substrate sites sandwiching an asymmetric four-base sequence, and NiDE cleavage resulted in 14-base single-stranded termini at both e...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 49(2010), 5 vom: 15. Nov., Seite 817-21
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| 1. Verfasser: |
Yang, Jie
(VerfasserIn) |
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| Weitere Verfasser: |
Zhang, Zhihong,
Zhang, Xin A,
Luo, Qingming |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Bacterial Proteins
DNA, Complementary
Luminescent Proteins
yellow fluorescent protein, Bacteria
DNA
9007-49-2
Deoxyribonuclease I
EC 3.1.21.1 |
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