Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes
To enable studies of conformational changes within multimolecular complexes, we present a simultaneous, four-color single molecule fluorescence methodology implemented with total internal reflection illumination and camera-based, wide-field detection. We further demonstrate labeling histidine-tagged...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1993. - 49(2010), 5 vom: 15. Nov., Seite 807-16
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| Auteur principal: |
DeRocco, Vanessa
(Auteur) |
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| Autres auteurs: |
Anderson, Trevor,
Piehler, Jacob,
Erie, Dorothy A,
Weninger, Keith |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2010
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Coloring Agents
Macromolecular Substances
SNARE Proteins
Tromethamine
023C2WHX2V
Histidine
4QD397987E
plus...
DNA
9007-49-2
MutS DNA Mismatch-Binding Protein
EC 3.6.1.3
Nitrilotriacetic Acid
KA90006V9D |
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