Generation of nonvernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis

© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 189(2011), 2 vom: 15. Jan., Seite 409-14
Auteur principal: Lochlainn, Seosamh O (Auteur)
Autres auteurs: Fray, Rupert G, Hammond, John P, King, Graham J, White, Philip J, Young, Scott D, Broadley, Martin R
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't Metals, Heavy
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245 1 0 |a Generation of nonvernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis 
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500 |a CommentIn: New Phytol. 2011 Jan;189(2):357-9. doi: 10.1111/j.1469-8137.2010.03591.x. - PMID 21175629 
500 |a Citation Status MEDLINE 
520 |a © 2010 The Authors. New Phytologist © 2010 New Phytologist Trust. 
520 |a Noccaea caerulescens (formerly Thlaspi caerulescens) is a widely studied metal hyperaccumulator. However, molecular genetic studies are challenging in this species because of its vernal-obligate biennial life cycle of 7-9months. Here, we describe the development of genetically stable, faster cycling lines of N. caerulescens which are nonvernal-obligate. A total of 5500 M(0) seeds from Saint Laurent Le Minier (France) were subjected to fast neutron mutagenesis. Following vernalization of young plants, 79% of plants survived to maturity. In all, 80,000 M(2) lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with nine flowering in <12wk. Two lines (A2 and A7) were selfed to the M(4) generation. Floral initials were observed 66 and 87d after sowing (DAS) in A2 and A7, respectively. Silicle development occurred for all A2 and for most A7 at 92 and 123 DAS, respectively. Floral or silicle development was not observed in wild-type (WT) plants. Leaf zinc (Zn) concentration was similar in WT, A2 and A7 lines. These lines should facilitate future genetic studies of this remarkable species. Seed is publicly available through the European Arabidopsis Stock Centre (NASC) 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Metals, Heavy  |2 NLM 
700 1 |a Fray, Rupert G  |e verfasserin  |4 aut 
700 1 |a Hammond, John P  |e verfasserin  |4 aut 
700 1 |a King, Graham J  |e verfasserin  |4 aut 
700 1 |a White, Philip J  |e verfasserin  |4 aut 
700 1 |a Young, Scott D  |e verfasserin  |4 aut 
700 1 |a Broadley, Martin R  |e verfasserin  |4 aut 
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