Cross-talk interactions of sucrose and Fusarium oxysporum in the phenylpropanoid pathway and the accumulation and localization of flavonoids in embryo axes of yellow lupine

Copyright © 2010 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 168(2011), 5 vom: 15. März, Seite 424-33
1. Verfasser: Morkunas, Iwona (VerfasserIn)
Weitere Verfasser: Narożna, Dorota, Nowak, Witold, Samardakiewicz, Sławomir, Remlein-Starosta, Dorota
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Flavonoids Phenylpropionates RNA, Messenger Sucrose 57-50-1 phenylpropiolic acid 637-44-5 mehr... Intramolecular Lyases EC 5.5.- chalcone isomerase EC 5.5.1.6
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245 1 0 |a Cross-talk interactions of sucrose and Fusarium oxysporum in the phenylpropanoid pathway and the accumulation and localization of flavonoids in embryo axes of yellow lupine 
264 1 |c 2011 
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520 |a Copyright © 2010 Elsevier GmbH. All rights reserved. 
520 |a This study investigated the effects of cross-talk interactions of sucrose and infection caused by a pathogenic fungus Fusarium oxysporum f.sp. lupini on the regulation of the phenylpropanoid pathway, i.e. the level of expression of genes encoding enzymes participating in flavonoid biosynthesis, as well as cell location and accumulation of these compounds in embryo axes of Lupinus luteus L. cv. Polo. Embryo axes, both non-inoculated and inoculated, were cultured for 96h on Heller medium with 60mM sucrose (+Sn and +Si) or without it (-Sn and -Si). Real-time RT-PCR to assess expression levels of the flavonoid biosynthetic genes, phenylalanine ammonialyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI) and isoflavone synthase (IFS) were used. Sucrose alone strongly stimulated the expression of these genes. There was a very high expression level of these genes in +Si embryo axes in the early phase of infection. Signal amplification by sucrose and the infection was most intense in the 48-h +Si axes, resulting in the highest level of expression of flavonoid biosynthetic genes. In -Si tissues, the expression level of these genes increased at 48 and 72h after inoculation relative to 24h; however, the relative level of expression was much lower than in +Si axes, except at 72h for PAL and CHS.Moreover, at 48h of culture, considerably higher activity of CHI (EC 5.5.1.6) was observed in axes with a high level of sucrose than in those with a sucrose deficit. CHI activity in +Si axes at 48 and 96h post-inoculation was over 1.5 and 2 times higher than that in +Sn axes, as well as higher than in -Si axes.Observations of yellow lupine embryo axes under a confocal microscope showed an increased post-infection accumulation of flavonoids, particularly in cells of embryo axes infected with F. oxysporum and cultured on a medium containing sucrose (+Si). Up to 48h post-infection in +Si axes, a very intensive emission of green fluorescence was observed, indicating high accumulation of these compounds in whole cells. Moreover, a nuclear location of flavonoids was recorded in cells. Strong staining of flavonoid end products in +Si embryo axes was consistent with the expression of PAL, CHS, CHI and IFS.These results indicate that, in the early phase of infection, the flavonoid biosynthesis pathway is considerably enhanced in yellow lupine embryo axes as a strong signal amplification effect of sucrose and the pathogenic fungus F. oxysporum 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a DNA Primers  |2 NLM 
650 7 |a Flavonoids  |2 NLM 
650 7 |a Phenylpropionates  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a Sucrose  |2 NLM 
650 7 |a 57-50-1  |2 NLM 
650 7 |a phenylpropiolic acid  |2 NLM 
650 7 |a 637-44-5  |2 NLM 
650 7 |a Intramolecular Lyases  |2 NLM 
650 7 |a EC 5.5.-  |2 NLM 
650 7 |a chalcone isomerase  |2 NLM 
650 7 |a EC 5.5.1.6  |2 NLM 
700 1 |a Narożna, Dorota  |e verfasserin  |4 aut 
700 1 |a Nowak, Witold  |e verfasserin  |4 aut 
700 1 |a Samardakiewicz, Sławomir  |e verfasserin  |4 aut 
700 1 |a Remlein-Starosta, Dorota  |e verfasserin  |4 aut 
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856 4 0 |u http://dx.doi.org/10.1016/j.jplph.2010.08.017  |3 Volltext 
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