pH-reversible, high-capacity binding of proteins on a substrate with nanostructure
In this letter, a pH-switchable system for protein adsorption and release is introduced. By combining the pH sensitivity of poly(methacrylic acid) (poly(MAA) chains and the nanoeffects of 3D nanostructured silicon nanowire arrays (SiNWAs), a poly(MAA)-modified SiNWAs material showed an extremely hig...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 26(2010), 23 vom: 07. Dez., Seite 17812-5 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Enzymes, Immobilized Polymethacrylic Acids Proteins polymethacrylic acid 25087-26-7 Muramidase EC 3.2.1.17 |
| Zusammenfassung: | In this letter, a pH-switchable system for protein adsorption and release is introduced. By combining the pH sensitivity of poly(methacrylic acid) (poly(MAA) chains and the nanoeffects of 3D nanostructured silicon nanowire arrays (SiNWAs), a poly(MAA)-modified SiNWAs material showed an extremely high capacity for binding lysozyme at pH 4 (an ∼80-fold increase compared with that of smooth Si-poly(MAA)). Moreover, ∼90% of the adsorbed lysozyme was released from SiNWAs-poly(MAA) by increasing the pH from 4 to 9, without a loss of enzyme activity |
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| Beschreibung: | Date Completed 06.04.2011 Date Revised 30.11.2010 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la103647s |