Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing
Here we describe a method for constructing small RNA libraries for high-throughput sequencing in which we have made a significant improvement to commonly available standard protocols. We added a locked nucleic acid (LNA) oligonucleotide--named dimer eliminator--that is complementary to the adapter-d...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 49(2010), 4 vom: 01. Okt., Seite 751-5
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| 1. Verfasser: |
Kawano, Mitsuoki
(VerfasserIn) |
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| Weitere Verfasser: |
Kawazu, Chika,
Lizio, Marina,
Kawaji, Hideya,
Carninci, Piero,
Suzuki, Harukazu,
Hayashizaki, Yoshihide |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA, Complementary
Oligonucleotides
locked nucleic acid
RNA
63231-63-0 |
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