One-step split GFP staining for sensitive protein detection and localization in mammalian cells
Although epitope tags are useful to detect intracellular proteins and follow their localization with antibodies, background and nonspecific staining often remain problematic. We describe a simple assay based on the split GFP complementation system. Proteins tagged with the 15-amino acid GFP 11 fragm...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 49(2010), 4 vom: 01. Okt., Seite 727-8, 730, 732 passim
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| Auteur principal: |
Kaddoum, Lara
(Auteur) |
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| Autres auteurs: |
Magdeleine, Eddy,
Waldo, Geoffrey S,
Joly, Etienne,
Cabantous, Stéphanie |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2010
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, Non-U.S. Gov't
Recombinant Fusion Proteins
Green Fluorescent Proteins
147336-22-9 |
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