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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la1036579
|2 doi
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|a pubmed25n0675.xml
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|a (DE-627)NLM202543668
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|a (NLM)20964389
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Madwar, Carolin
|e verfasserin
|4 aut
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|a Perfluorophenyl azide immobilization chemistry for single molecule force spectroscopy of the concanavalin A/mannose interaction
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 04.03.2011
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomolecules and polymers to surfaces. Herein, a single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded an average unbinding force of 70-80 pN for loading rates between 8000 and 40,000 pN/s for mannose surfaces on aminated glass, and an unbinding force of 57 ± 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k(off), for this interaction to be 0.16 s(-1)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Azides
|2 NLM
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|a Hydrocarbons, Fluorinated
|2 NLM
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|a perfluorophenyl azide
|2 NLM
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|a Concanavalin A
|2 NLM
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|a 11028-71-0
|2 NLM
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|a Mannose
|2 NLM
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|a PHA4727WTP
|2 NLM
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1 |
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|a Kwan, William Chu
|e verfasserin
|4 aut
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1 |
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|a Deng, Lingquan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ramström, Olof
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schmidt, Rolf
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zou, Shan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cuccia, Louis A
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 26(2010), 22 vom: 16. Nov., Seite 16677-80
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:26
|g year:2010
|g number:22
|g day:16
|g month:11
|g pages:16677-80
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|u http://dx.doi.org/10.1021/la1036579
|3 Volltext
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