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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la103542y
|2 doi
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|a pubmed24n0675.xml
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|a eng
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| 100 |
1 |
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|a Lee, Jiyeon
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Stamp wound assay for studying coupled cell migration and cell debris clearance
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 04.03.2011
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|a Date Revised 25.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Dimethylpolysiloxanes
|2 NLM
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| 650 |
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|a Heterocyclic Compounds, 4 or More Rings
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|a blebbistatin
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|a baysilon
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|a Myosin Type II
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| 700 |
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|a Wang, Yu-Lin
|e verfasserin
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|a Ren, Fan
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| 700 |
1 |
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|a Lele, Tanmay P
|e verfasserin
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 22 vom: 16. Nov., Seite 16672-6
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|x 1520-5827
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|g volume:26
|g year:2010
|g number:22
|g day:16
|g month:11
|g pages:16672-6
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|u http://dx.doi.org/10.1021/la103542y
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