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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2010.09.012
|2 doi
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|a pubmed25n0675.xml
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|a DE-627
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|a eng
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|a Troncoso-Ponce, M Adrián
|e verfasserin
|4 aut
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|a Glycolytic enzymatic activities in developing seeds involved in the differences between standard and low oil content sunflowers (Helianthus annuus L.)
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|c 2010
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.05.2011
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2010 Elsevier Masson SAS. All rights reserved.
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|a As opposed to other oilseeds, developing sunflower seeds do not accumulate starch initially. They rely on the sucrose that comes from the mother plant to synthesise lipid precursors. Glycolysis is the principal source of carbon skeletons and reducing power for lipid biosynthesis. In this work, glycolytic initial metabolites and enzyme activities from developing seed of two different sunflower lines, of high and low oil content, were compared during storage lipid synthesis. These two lines showed different kinetic lipid accumulation in the developing embryos. Fatty acids levels during the initial and final stage of lipid synthesis were higher in CAS-6 than in ZEN-8. The analysis of the photosynthate and sugars content suggests that, although the hexoses levels were quite similar in both lines, the amount of sucrose produced by the mother plant and available for lipid synthesis was higher in CAS-6. Although, a smaller amount of sucrose is available in the ZEN-8 line, its seeds maintain the levels of intermediate sugars in the initial steps of glycolysis due to an increase in the levels of the invertase, hexokinase and phosphoglucose isomerase activities in ZEN-8, with respect to CAS-6. Also, a readjustment in the final part of this metabolic route took place, with the activities of phosphoglycerate kinase and enolase in CAS-6 being higher, allowing increased synthesis of phosphoenolpiruvate, the intermediate carbon donor for fatty acid synthesis. In addition, recently, it has been shown that Arabidopsis mutants with a lower fat content in their seeds have a higher amount of sucrose. These data together point to these last two enzymatic activities, phosphoglycerate kinase and enolase, as being responsible for the lower fat content in the ZEN-8 line
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fatty Acids
|2 NLM
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|a Plant Oils
|2 NLM
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|a Sunflower Oil
|2 NLM
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|a Sucrose
|2 NLM
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|a 57-50-1
|2 NLM
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|a Phosphoglycerate Kinase
|2 NLM
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| 650 |
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|a EC 2.7.2.3
|2 NLM
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| 650 |
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|a Phosphopyruvate Hydratase
|2 NLM
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| 650 |
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|a EC 4.2.1.11
|2 NLM
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| 700 |
1 |
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|a Garcés, Rafael
|e verfasserin
|4 aut
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| 700 |
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|a Martínez-Force, Enrique
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 48(2010), 12 vom: 09. Dez., Seite 961-5
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
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|g volume:48
|g year:2010
|g number:12
|g day:09
|g month:12
|g pages:961-5
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|u http://dx.doi.org/10.1016/j.plaphy.2010.09.012
|3 Volltext
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