Mapping of an anthocyanin-regulating MYB transcription factor and its expression in red and green pear, Pyrus communis

Copyright © 2010 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 48(2010), 12 vom: 09. Dez., Seite 1020-6
1. Verfasser: Pierantoni, Luca (VerfasserIn)
Weitere Verfasser: Dondini, Luca, De Franceschi, Paolo, Musacchi, Stefano, Winkel, Brenda S J, Sansavini, Silviero
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Anthocyanins Transcription Factors
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520 |a Copyright © 2010 Elsevier Masson SAS. All rights reserved. 
520 |a 'Max Red Bartlett' is a red bud mutation of the yellow pear (Pyrus communis L.) cultivar 'Williams' (known as 'Bartlett' in North America). Anthocyanins are the most important pigments for red colour in fruits. Synthesis of anthocyanins is mediated by a number of well-characterized enzymes that include chalcone synthase (CHS), flavanone-3-hydroxylase (F3H), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), and UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT). Expression of the genes encoding these five enzymes was examined in pear fruit skin in order to elucidate the molecular mechanism for red coloration. In addition, the gene PcMYB10, encoding an R2R3 MYB transcription factor involved in anthocyanin biosynthetic pathway regulation, was isolated from both 'Williams' and 'Max Red Bartlett'. Analysis of the deduced amino acid sequence suggests that this gene is an ortholog of anthocyanin regulators known in other plant species. Its expression level was significantly higher in 'Max Red Bartlett' (red pear) compared with the original yellow variety 'Williams'. Although the map position of PcMYB10 corresponds to that of MdMYBa and MdMYB10, which control pigmentation of apple fruit skin, PcMYB10 is not directly responsible for red versus yellow colour in the two pear varieties, as the mutation underlying this difference maps to a different region of the pear genome 
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650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Anthocyanins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
700 1 |a Dondini, Luca  |e verfasserin  |4 aut 
700 1 |a De Franceschi, Paolo  |e verfasserin  |4 aut 
700 1 |a Musacchi, Stefano  |e verfasserin  |4 aut 
700 1 |a Winkel, Brenda S J  |e verfasserin  |4 aut 
700 1 |a Sansavini, Silviero  |e verfasserin  |4 aut 
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