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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2010.09.003
|2 doi
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|a pubmed24n0674.xml
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|a (DE-627)NLM20225111X
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|a (NLM)20933298
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
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|a Eltelib, Hani A
|e verfasserin
|4 aut
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|a Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra)
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.05.2011
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|a Date Revised 10.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2010 Elsevier GmbH. All rights reserved.
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|a Acerola (Malpighia glabra) is an exotic fruit cultivated primarily for its abundant ascorbic acid (AsA) content. The molecular mechanisms that regulate the metabolism of AsA in acerola have yet to be defined. Monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) are key enzymes of the ascorbate-glutathione cycle that maintain reduced pools of ascorbic acid and serve as important antioxidants. cDNAs encoding MDHAR and DHAR were isolated from acerola using RT-PCR and RACE. Phylogenetic trees associated acerola MDHAR and DHAR with other plant cytosolic MDHARs and DHARs. Expressions of the two genes correlated with their enzymatic activities and were differentially regulated during fruit ripening. Interestingly, MDHAR expression was only detected in overripe fruits, whereas the transcript level of DHAR was highest at the intermediate stage of fruit ripening. Under dark conditions, there was a sharp and significant decline in the total and reduced ascorbate contents, accompanied by a decrease in the level of transcripts and enzyme activities of the two genes in acerola leaves. MDHAR and DHAR transcripts and enzyme activities were significantly up-regulated in the leaves of acerola under cold and salt stress conditions, indicating that expression of both genes are transcriptionally regulated under these stresses
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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|a Plant Proteins
|2 NLM
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|a RNA, Messenger
|2 NLM
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|a Oxidoreductases
|2 NLM
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|a EC 1.-
|2 NLM
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|a NADH, NADPH Oxidoreductases
|2 NLM
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|a EC 1.6.-
|2 NLM
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|a monodehydroascorbate reductase (NADH)
|2 NLM
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| 650 |
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|a EC 1.6.5.4
|2 NLM
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| 650 |
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|a glutathione dehydrogenase (ascorbate)
|2 NLM
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| 650 |
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|a EC 1.8.5.1
|2 NLM
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| 650 |
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|a Ascorbic Acid
|2 NLM
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| 650 |
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|a PQ6CK8PD0R
|2 NLM
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| 700 |
1 |
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|a Badejo, Adebanjo A
|e verfasserin
|4 aut
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|a Fujikawa, Yukichi
|e verfasserin
|4 aut
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| 700 |
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|a Esaka, Muneharu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 6 vom: 15. Apr., Seite 619-27
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:6
|g day:15
|g month:04
|g pages:619-27
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|u http://dx.doi.org/10.1016/j.jplph.2010.09.003
|3 Volltext
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|d 168
|j 2011
|e 6
|b 15
|c 04
|h 619-27
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