Nonrigid registration of 2-D and 3-D dynamic cell nuclei images for improved classification of subcellular particle motion

The observed motion of subcellular particles in fluorescence microscopy image sequences of live cells is generally a superposition of the motion and deformation of the cell and the motion of the particles. Decoupling the two types of movements to enable accurate classification of the particle motion...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:IEEE transactions on image processing : a publication of the IEEE Signal Processing Society. - 1992. - 20(2011), 4 vom: 01. Apr., Seite 1011-22
1. Verfasser: Kim, Il-Han (VerfasserIn)
Weitere Verfasser: Chen, Yi-Chun M, Spector, David L, Eils, Roland, Rohr, Karl
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:IEEE transactions on image processing : a publication of the IEEE Signal Processing Society
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
LEADER 01000caa a22002652 4500
001 NLM201562677
003 DE-627
005 20250212001856.0
007 cr uuu---uuuuu
008 231223s2011 xx |||||o 00| ||eng c
024 7 |a 10.1109/TIP.2010.2076377  |2 doi 
028 5 2 |a pubmed25n0672.xml 
035 |a (DE-627)NLM201562677 
035 |a (NLM)20840894 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Kim, Il-Han  |e verfasserin  |4 aut 
245 1 0 |a Nonrigid registration of 2-D and 3-D dynamic cell nuclei images for improved classification of subcellular particle motion 
264 1 |c 2011 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 16.08.2011 
500 |a Date Revised 20.10.2021 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a The observed motion of subcellular particles in fluorescence microscopy image sequences of live cells is generally a superposition of the motion and deformation of the cell and the motion of the particles. Decoupling the two types of movements to enable accurate classification of the particle motion requires the application of registration algorithms. We have developed an intensity-based approach for nonrigid registration of multichannel microscopy image sequences of cell nuclei. First, based on 3-D synthetic images we demonstrate that cell nucleus deformations change the observed motion types of particles and that our approach allows to recover the original motion. Second, we have successfully applied our approach to register 2-D and 3-D real microscopy image sequences. A quantitative experimental comparison with previous approaches for nonrigid registration of cell microscopy has also been performed 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
700 1 |a Chen, Yi-Chun M  |e verfasserin  |4 aut 
700 1 |a Spector, David L  |e verfasserin  |4 aut 
700 1 |a Eils, Roland  |e verfasserin  |4 aut 
700 1 |a Rohr, Karl  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t IEEE transactions on image processing : a publication of the IEEE Signal Processing Society  |d 1992  |g 20(2011), 4 vom: 01. Apr., Seite 1011-22  |w (DE-627)NLM09821456X  |x 1941-0042  |7 nnns 
773 1 8 |g volume:20  |g year:2011  |g number:4  |g day:01  |g month:04  |g pages:1011-22 
856 4 0 |u http://dx.doi.org/10.1109/TIP.2010.2076377  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 20  |j 2011  |e 4  |b 01  |c 04  |h 1011-22