Molecular characterization of RsMPK2, a C1 subgroup mitogen-activated protein kinase in the desert plant Reaumuria soongorica

Copyright © 2010 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 48(2010), 10-11 vom: 02. Okt., Seite 836-44
1. Verfasser: Liu, Yubing (VerfasserIn)
Weitere Verfasser: Li, Xinrong, Tan, Huijuan, Liu, Meiling, Zhao, Xin, Wang, Jin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antioxidants Butadienes DNA, Complementary Nitriles Plant Proteins U 0126 Abscisic Acid 72S9A8J5GW mehr... Hydrogen Peroxide BBX060AN9V Mitogen-Activated Protein Kinases EC 2.7.11.24
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520 |a Reaumuria soongorica (Pall.) Maxim. is a short woody shrub widely found in semi-arid areas of China, and can survive severe environmental stresses. To understand its potential signaling transduction pathway in stress tolerance, we investigated the participation of mitogen-activated protein kinases (MAPKs) as possible mediators of abiotic stresses. A novel MAP kinase cDNA (RsMPK2) that encodes a 374 amino acid protein was isolated from R. soongorica. RsMPK2 belongs to the C1 subgroup, which is still functionally uncharacterized compared to groups A and B; and contains all 11 of the conserved MAPK subdomains and the TEY phosphorylation motif. RsMPK2 is expressed in vegetative (root, stem, leaf and callus) and reproductive (flower) organs. The transcripts of RsMPK2 were rapidly accumulated at high levels when R. soongorica was subjected to dehydration, salinity conditions and treatment with abscisic acid or hydrogen peroxide. Growth analysis of Escherichia coli (srl::Tn10) cells transformed with pPROEXHT-RsMPK2 showed that the expression products of RsMPK2 do not act as an osmoprotectant. But, the inhibition of RsMPK2 expression by the inhibitor U0126 induced a decrease of antioxidant enzyme activity under stresses, indicating that RsMPK2 is involved in the regulation of the antioxidant defense system in the response to stress signaling 
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650 7 |a Nitriles  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a U 0126  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
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700 1 |a Li, Xinrong  |e verfasserin  |4 aut 
700 1 |a Tan, Huijuan  |e verfasserin  |4 aut 
700 1 |a Liu, Meiling  |e verfasserin  |4 aut 
700 1 |a Zhao, Xin  |e verfasserin  |4 aut 
700 1 |a Wang, Jin  |e verfasserin  |4 aut 
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