Involvement of anion channels in mediating elicitor-induced ATP efflux in Salvia miltiorrhiza hairy roots

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 168(2011), 2 vom: 15. Jan., Seite 128-32
1. Verfasser:	Wu, Shu-Jing (VerfasserIn)
Weitere Verfasser:	Siu, Ka-Chai, Wu, Jian-Yong
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2011
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't ATP-Binding Cassette Transporters Ion Channels Adenosine Triphosphate 8L70Q75FXE

Beschreibung
Zusammenfassung:	Copyright © 2010 Elsevier GmbH. All rights reserved. This study examines the roles of anion channels and ATP binding cassette (ABC) protein transporters in mediating elicitor-induced ATP release in Salvia miltiorrhiza hairy root cultures. The elicitor-induced ATP release was effectively blocked by two putative membrane anion channel blockers, niflumic acid and Zn(2+), but not by a specific Cl(-) channel blocker, phenylanthranilic acid. The elicitor-induced ATP release was also significantly suppressed by two ABC inhibitors, glibenclamide and ethacrynic acid. Notable ATP release from the hairy roots was also induced by verapamil (2mM), an ABC activator in animal cells. The verapamil-induced ATP release was effectively blocked by niflumic acid, but only slightly inhibited by the ABC inhibitors. Another notable effect of verapamil was the induction of exocytosis, the secretion of vesicle-like particles to the root surface. The verapamil-induced exocytosis was not inhibited by nifulumic acid and YE did not induce the exocytosis. Overall, the results suggest a significant role of anion channels, a possible involvement of ABC proteins and no significant involvement of exocytosis in mediating the ATP efflux in hairy root cells
Beschreibung:	Date Completed 07.04.2011 Date Revised 30.09.2020 published: Print Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2010.07.015