Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation

© The Authors (2010). Journal compilation © New Phytologist Trust (2010).

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 188(2010), 4 vom: 23. Dez., Seite 985-1000
1. Verfasser: Stracke, Ralf (VerfasserIn)
Weitere Verfasser: Jahns, Oliver, Keck, Matthias, Tohge, Takayuki, Niehaus, Karsten, Fernie, Alisdair R, Weisshaar, Bernd
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis Proteins Flavonols Glycosides MYB11 protein, Arabidopsis MYB12 protein, Arabidopsis Transcription Factors Glucuronosyltransferase EC 2.4.1.17
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245 1 0 |a Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation 
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520 |a © The Authors (2010). Journal compilation © New Phytologist Trust (2010). 
520 |a The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs production of flavonol glycoside1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen 
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650 7 |a Flavonols  |2 NLM 
650 7 |a Glycosides  |2 NLM 
650 7 |a MYB11 protein, Arabidopsis  |2 NLM 
650 7 |a MYB12 protein, Arabidopsis  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Glucuronosyltransferase  |2 NLM 
650 7 |a EC 2.4.1.17  |2 NLM 
700 1 |a Jahns, Oliver  |e verfasserin  |4 aut 
700 1 |a Keck, Matthias  |e verfasserin  |4 aut 
700 1 |a Tohge, Takayuki  |e verfasserin  |4 aut 
700 1 |a Niehaus, Karsten  |e verfasserin  |4 aut 
700 1 |a Fernie, Alisdair R  |e verfasserin  |4 aut 
700 1 |a Weisshaar, Bernd  |e verfasserin  |4 aut 
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