Cloning and expression analysis of cDNAs for ABA 8'-hydroxylase during sweet cherry fruit maturation and under stress conditions

Copyright © 2010 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 167(2010), 17 vom: 15. Nov., Seite 1486-93
1. Verfasser: Ren, Jie (VerfasserIn)
Weitere Verfasser: Sun, Liang, Wu, Jiefang, Zhao, Shengli, Wang, Canlei, Wang, Yanping, Ji, Kai, Leng, Ping
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article DNA, Complementary Plant Proteins Abscisic Acid 72S9A8J5GW Cytochrome P-450 Enzyme System 9035-51-2 Dioxygenases EC 1.13.11.- 9-cis-epoxy-carotenoid dioxygenase mehr... EC 1.13.11.51 abscisic acid 8'-hydroxylase EC 1.14.14.137
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245 1 0 |a Cloning and expression analysis of cDNAs for ABA 8'-hydroxylase during sweet cherry fruit maturation and under stress conditions 
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520 |a Abscisic acid (ABA) plays a key role in various aspects of plant growth and development, including adaptation to environmental stress and fruit maturation in sweet cherry fruit. In higher plants, the level of ABA is determined by synthesis and catabolism. In order to gain insight into ABA synthesis and catabolism in sweet cherry fruit during maturation and under stress conditions, four cDNAs of PacCYP707A1 -PacCYP707A4 for 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA, and one cDNA of PacNCED1 for 9-cis-epoxycarotenoid dioxygenase, a key enzyme in the ABA biosynthetic pathway, were isolated from sweet cherry fruit (Prunus avium L.). The timing and pattern of PacNCED1 expression was coincident with that of ABA accumulation, which was correlated to maturation of sweet cherry fruit. All four PacCYP707As were expressed at varying intensities throughout fruit development and appeared to play overlapping roles in ABA catabolism throughout sweet cherry fruit development. The application of ABA enhanced the expression of PacCYP707A1 -PacCYP707A3 as well as PacNCED1, but downregulated the PacCYP707A4 transcript level. Expressions of PacCYP707A1, PacCYP707A3 and PacNCED1 were strongly increased by water stress. No significant differences in PacCYP707A2 and PacCYP707A4 expression were observed between dehydrated and control fruits. The results suggest that endogenous ABA content is modulated by a dynamic balance between biosynthesis and catabolism, which are regulated by PacNCED1 and PacCYP707As transcripts, respectively, during fruit maturation and under stress conditions 
650 4 |a Journal Article 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
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650 7 |a 9035-51-2  |2 NLM 
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650 7 |a 9-cis-epoxy-carotenoid dioxygenase  |2 NLM 
650 7 |a EC 1.13.11.51  |2 NLM 
650 7 |a abscisic acid 8'-hydroxylase  |2 NLM 
650 7 |a EC 1.14.14.137  |2 NLM 
700 1 |a Sun, Liang  |e verfasserin  |4 aut 
700 1 |a Wu, Jiefang  |e verfasserin  |4 aut 
700 1 |a Zhao, Shengli  |e verfasserin  |4 aut 
700 1 |a Wang, Canlei  |e verfasserin  |4 aut 
700 1 |a Wang, Yanping  |e verfasserin  |4 aut 
700 1 |a Ji, Kai  |e verfasserin  |4 aut 
700 1 |a Leng, Ping  |e verfasserin  |4 aut 
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