Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense

The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized....

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Veröffentlicht in:Environmental technology. - 1998. - 31(2010), 10 vom: 14. Sept., Seite 1083-90
1. Verfasser: Kluskens, L D (VerfasserIn)
Weitere Verfasser: Zeilstra, J, Geerling, A C M, de Vos, W M, van der Oost, J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Bacterial Proteins Recombinant Proteins Aldose-Ketose Isomerases EC 5.3.1.- xylose isomerase EC 5.3.1.5
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520 |a The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized. It is optimally active at 70 degrees C, pH 7.3, with a specific activity of 15.0 U/mg for the interconversion of glucose to fructose. When compared with T. maritima XylA at 85 degrees C, a higher catalytic efficiency was observed. Divalent metal ions Co2+ and Mg2+ were found to enhance the thermostability 
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700 1 |a Geerling, A C M  |e verfasserin  |4 aut 
700 1 |a de Vos, W M  |e verfasserin  |4 aut 
700 1 |a van der Oost, J  |e verfasserin  |4 aut 
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