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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1080/09593330903486673
|2 doi
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|a pubmed25n0668.xml
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|a (DE-627)NLM200389246
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|a (NLM)20718290
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|e rakwb
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|a eng
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1 |
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|a Kluskens, L D
|e verfasserin
|4 aut
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|a Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 16.09.2010
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|a Date Revised 17.11.2011
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|a published: Print
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|a Citation Status MEDLINE
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|a The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized. It is optimally active at 70 degrees C, pH 7.3, with a specific activity of 15.0 U/mg for the interconversion of glucose to fructose. When compared with T. maritima XylA at 85 degrees C, a higher catalytic efficiency was observed. Divalent metal ions Co2+ and Mg2+ were found to enhance the thermostability
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|a Journal Article
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|a Bacterial Proteins
|2 NLM
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|a Recombinant Proteins
|2 NLM
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|a Aldose-Ketose Isomerases
|2 NLM
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| 650 |
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|a EC 5.3.1.-
|2 NLM
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|a xylose isomerase
|2 NLM
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| 650 |
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|a EC 5.3.1.5
|2 NLM
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| 700 |
1 |
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|a Zeilstra, J
|e verfasserin
|4 aut
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1 |
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|a Geerling, A C M
|e verfasserin
|4 aut
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1 |
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|a de Vos, W M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a van der Oost, J
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Environmental technology
|d 1998
|g 31(2010), 10 vom: 14. Sept., Seite 1083-90
|w (DE-627)NLM098202545
|x 0959-3330
|7 nnns
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| 773 |
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|g volume:31
|g year:2010
|g number:10
|g day:14
|g month:09
|g pages:1083-90
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|u http://dx.doi.org/10.1080/09593330903486673
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