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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la102616h
|2 doi
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|a pubmed25n0668.xml
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|a (DE-627)NLM200292773
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|a (NLM)20707324
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Tiemeyer, Sebastian
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Effect of surface charge distribution on the adsorption orientation of proteins to lipid monolayers
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.12.2010
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|a Date Revised 15.11.2012
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|a published: Print
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|a Citation Status MEDLINE
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|a The adsorption orientation of the proteins lysozyme and ribonuclease A (RNase A) to a neutral 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and a negatively charged stearic acid lipid film was investigated by means of X-ray reflectivity. Both proteins adsorbed to the negatively charged lipid monolayer, whereas at the neutral monolayer, no adsorption was observed. For acquiring comprehensive information on the proteins' adsorption, X-ray reflectivity data were combined with electron densities obtained from crystallographic data. With this method, it is possible to determine the orientation of adsorbed proteins in solution underneath lipid monolayers. While RNase A specifically coupled with its positively charged active site to the negatively charged lipid monolayer, lysozyme prefers an orientation with its long axis parallel to the Langmuir film. In comparison to the electrostatic maps of the proteins, our results can be explained by the discriminative surface charge distribution of lysozyme and RNase A
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Membranes, Artificial
|2 NLM
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|a Stearic Acids
|2 NLM
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| 650 |
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|a 1,2-Dipalmitoylphosphatidylcholine
|2 NLM
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|a 2644-64-6
|2 NLM
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|a stearic acid
|2 NLM
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| 650 |
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|a 4ELV7Z65AP
|2 NLM
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| 650 |
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|a Ribonuclease, Pancreatic
|2 NLM
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| 650 |
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|a EC 3.1.27.5
|2 NLM
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| 650 |
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|a Muramidase
|2 NLM
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| 650 |
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|a EC 3.2.1.17
|2 NLM
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| 700 |
1 |
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|a Paulus, Michael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tolan, Metin
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 26(2010), 17 vom: 07. Sept., Seite 14064-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:26
|g year:2010
|g number:17
|g day:07
|g month:09
|g pages:14064-7
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|u http://dx.doi.org/10.1021/la102616h
|3 Volltext
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