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231223s2010 xx |||||o 00| ||eng c |
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|a 10.2144/000113465
|2 doi
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|a eng
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1 |
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|a Huang, Wei-Hong
|e verfasserin
|4 aut
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|a Modified agarose gel for high-performance Southern blotting of short DNA fragments
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|c 2010
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|a Text
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|a ƒaComputermedien
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|a Date Completed 30.03.2011
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|a Date Revised 12.08.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolved fractionation of short DNA fragments comparable to that from a high-concentration agarose gel could be obtained using a low-concentration agarose gel with a small zone of high-concentration agarose, and that the resulting DNA transfer is highly efficient and rapid
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Huang, Xiu-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Fang-Zhen
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 49(2010), 2 vom: 15. Aug., Seite 580-1
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
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|g volume:49
|g year:2010
|g number:2
|g day:15
|g month:08
|g pages:580-1
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|u http://dx.doi.org/10.2144/000113465
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