Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies
Here we describe a labeling technique for the covalent linkage of quantum dots to transmembrane receptors for single-molecule tracking. Our method combines the acyl carrier protein (ACP) technique with coenzyme A (CoA)-functionalized quantum dots to covalently attach quantum dots to ACP fusions of r...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 49(2010), 2 vom: 15. Aug., Seite 574-9
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| 1. Verfasser: |
Zelman-Femiak, Monika
(VerfasserIn) |
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| Weitere Verfasser: |
Wang, Kun,
Gromova, Kira V,
Knaus, Petra,
Harms, Gregory S |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Acyl Carrier Protein
Receptor, Parathyroid Hormone, Type 1
Receptors, Cell Surface
Bone Morphogenetic Protein Receptors, Type II
EC 2.7.11.30 |
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